Invitrogen

BMI-1 Monoclonal Antibody (1H6B10G7)

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12 Published Figures

10 References

View all (49) BMI-1 antibodies

Datasheet

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Protocols

Datasheet

Certificates

Protocols

Datasheet

Certificates

Protocols

Additional Information:

Published Figures (12)
Advanced Verification (1)

FIGURE: 1 / 13

BMI-1 Antibody (37-5400) in WB

Molecular cancer therapeutics 2018 - Published figure using BMI-1 monoclonal antibody (Product # 37-5400) in Western Blot

BMI-1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

BMI-1 Antibody in Immunoprecipitation (IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

BMI-1 Antibody — Figure 2. BMI1 depletion-mediated autophagy is ATP-dependent. (A) CP20 and OVCAR4 cells were treated with either, si-Control, si- BMI1 or vehicle control and PTC-209 (100 nM) for 48 h and intracellular ATP levels determined and normalized with the respective number of viable cells in each group and compared with respect to control. Data represent mean +- SD of 3 independent experiments performed in triplicate. * P < 0.05 when comparing si-Control vs si- BMI1 and #, P < 0.05 when comparing control vs PTC. (B) si-RNA-transfected (48 h) CP20 and OVCAR4 cells were supplemented with 2 uM ATP for the last 4 h. 10 uM FCCP for 4 h was used as a positive control. Expression of BMI1, MAP1LC3B-II, SQSTM1 and ACTB was determined by western blot. (C) CP20 and OVCAR4 cells were treated with PTC-209 (PTC) for 48 h at 100 nM and supplemented with 2 uM ATP for the last 4 h. Expression of BMI1, MAP1LC3B-II, SQSTM1 and ACTB was determined by western blot. (D) CP20 and OVCAR4 cells were treated with 100 nM of PTC-209 (PTC) for 48 h and western blots were performed with the respective indicated antibodies. {TM}

Product Details

37-5400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 8 publications 8 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1H6B10G7

Immunogen

Synthetic peptide derived from the mid region of the human Bim-1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Storage conditions

-20°C

Shipping conditions

Wet ice

AB_2533326

Target Information

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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BMI-1 Monoclonal Antibody (1H6B10G7)

BMI-1 Antibody (37-5400) in WB

Product Details

37-5400

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Storage conditions

Shipping conditions

RRID

Target Information

Performance Guarantee

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