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Invitrogen
DDB1 Monoclonal Antibody (2B12D1)
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FIGURE: 1 / 18
DDB1 Antibody (37-6200) in WB
Western blot analysis was performed on whole cell extracts of MCF7 (Lane 1), T-47D (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4), NIH/3T3 (Lane 5), Hep G2 (Lane 6). The blots were probed with Anti- DDB1 Mouse Monoclonal (Product # 37-6200, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~ 130 kDa band corresponding to DDB1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
37-6200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2B12D1
Immunogen
Recombinant human DDB1 protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: damage-specific DNA binding protein 1, 127kDa; damage-specific DNA-binding protein, DNA repair; damaged-DNA recognition protein 1; DDB p127 subunit; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; unnamed protein product; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XAP-1; xeroderma pigmento; xeroderma pigmentosum group E-complementing protein; XPE-binding factor
Gene Aliases: 127kDa; DDBA; p127-Ddb1; UV-DDB1; WHIKERS; XAP1; XPCE; XPE; XPE-BF
UniProt ID: (Human) Q16531, (Mouse) Q3U1J4
Entrez Gene ID: (Human) 1642, (Mouse) 13194
DNA repair
nucleotide-excision repair
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
spindle assembly involved in female meiosis
spermatogenesis
cell proliferation
proteasomal protein catabolic process
regulation of autophagy
protein ubiquitination
viral release from host cell
regulation of DNA-dependent DNA replication initiation
replication fork processing
regulation of natural killer cell activation
cellular response to UV
regulation of gene expression, epigenetic
regulation of cell proliferation
regulation of circadian rhythm
regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
epigenetic programming in the zygotic pronuclei
positive regulation of viral genome replication
positive regulation of gluconeogenesis
positive regulation of protein catabolic process
regulation of embryonic development
positive regulation by virus of viral protein levels in host cell
interaction with symbiont
regulation of gene silencing by miRNA
UV-damage excision repair
regulation of cellular response to stress
regulation of cell cycle phase transition
regulation of mitotic cell cycle phase transition
regulation of mitotic cytokinesis
negative regulation of adipose tissue development
regulation of stem cell population maintenance
Wnt signaling pathway
negative regulation of apoptotic process
negative regulation of protein kinase B signaling
type I interferon signaling pathway
base-excision repair, AP site formation via deaminated base removal
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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