Thermo Scientific™

Pierce™ Lane Marker Reducing Sample Buffer

Catalog number: 39000
Thermo Scientific™

Pierce™ Lane Marker Reducing Sample Buffer

Catalog number: 39000
Catalog Number
Unit Size
5 mL
Price (USD)
Price: 87.75
Your Price:
Catalog NumberUnit SizePrice (USD)Quantity
390005 mL
Price: 87.75
Your Price:
Product Overview
Citations & References
Additional Information
Thermo Scientific Pierce Lane Marker Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a reducing formulation, with a pink dye buffer-front marker. This Sample Buffer contains DTT as the reducing agent, eliminating the strong odor associated with mercaptoethanol-containing buffers.

Features of Lane Marker Sample Buffer:

Easy to see—bright pink dye marks the buffer front, clearly indicating the progress of the electrophoresis run
Concentrated—5X stocks enable a greater range of protein sample volumes to be used for electrophoresis
Transferable marker dye—pink dye transfers with protein to nitrocellulose membrane, enabling verification of transfer direction and orientation of the blot

Lane Marker Reducing Sample Buffer is convenient and ready to use for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Formulated as a 5X stock rather than the traditional 2X stock, it enables a larger volume of protein solution to be included in the sample that is loaded in each well. Particularly unique in this buffer is the use of a bright pink tracking dye instead of the traditional bromophenol blue dye. This pink dye marks not only the dye front in the gel but also transfers and permanently binds to nitrocellulose membranes. Consequently, the dye can be used to assess transfer efficiency and align cut membranes.

Lane Marker Sample Buffer is easy to use. Simply mix one part Sample Buffer with four parts protein sample, heat denature, and then load the gel sample wells. Visualize progress of the electrophoresis run by monitoring the location of the pink dye front. Lane Marker Sample Buffer may be used in denaturing gels of all kinds and is compatible with coomassie dye and silver staining techniques, as well as Western blotting procedures.

Note: This product is not compatible with fluorescent detection systems. (The pink tracking dye fluoresces strongly.)

Related Products
Pierce™ Lane Marker Non-Reducing Sample Buffer
For Research Use Only. Not for use in diagnostic procedures.


Pierce Lane Marker Reducing Sample Buffer (5X)
Sample Loading Buffers
Detection Method
Gel Type
Product Type
Lane Marker Reducing Sample Buffer
Gel Compatibility
Product Line

Contents & Storage

Store single-use aliquots at -5 to -30°C.


Documents & Downloads


    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text