Pierce™ Lane Marker Reducing Sample Buffer

Catalog number: 39000

Thermo Scientific™  Related applications: Protein Gel Electrophoresis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Thermo Scientific Pierce Lane Marker Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a reducing formulation, with a pink dye buffer-front marker. This Sample Buffer contains DTT as the reducing agent, eliminating the strong odor associated with mercaptoethanol-containing buffers.

Features of Lane Marker Sample Buffer:

Easy to see—bright pink dye marks the buffer front, clearly indicating the progress of the electrophoresis run
Concentrated—5X stocks enable a greater range of protein sample volumes to be used for electrophoresis
Transferable marker dye—pink dye transfers with protein to nitrocellulose membrane, enabling verification of transfer direction and orientation of the blot

Lane Marker Reducing Sample Buffer is convenient and ready to use for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Formulated as a 5X stock rather than the traditional 2X stock, it enables a larger volume of protein solution to be included in the sample that is loaded in each well. Particularly unique in this buffer is the use of a bright pink tracking dye instead of the traditional bromophenol blue dye. This pink dye marks not only the dye front in the gel but also transfers and permanently binds to nitrocellulose membranes. Consequently, the dye can be used to assess transfer efficiency and align cut membranes.

Lane Marker Sample Buffer is easy to use. Simply mix one part Sample Buffer with four parts protein sample, heat denature, and then load the gel sample wells. Visualize progress of the electrophoresis run by monitoring the location of the pink dye front. Lane Marker Sample Buffer may be used in denaturing gels of all kinds and is compatible with coomassie dye and silver staining techniques, as well as Western blotting procedures.

Note: This product is not compatible with fluorescent detection systems. (The pink tracking dye fluoresces strongly.)

Related Products
Pierce™ Lane Marker Non-Reducing Sample Buffer
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Type: SDS-PAGE Gel (any)
Buffer Type: Sample Loading Buffers
Concentrated: 5 X
Product Size: 5 mL
Gel Compatibility: SDS-PAGE Gels

Contents & storage

Upon receipt store at room temperature. Product is shipped at ambient temperature.

Documents

Manuals & protocols