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Invitrogen
ZO-1 Polyclonal Antibody (ZMD.437)
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FIGURE: 1 / 88
ZO-1 Antibody (40-2300) in ICC/IF
Immunofluorescent analysis of ZO-1 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 30 minutes at room temperature. Cells were stained with or without ZO-1 rabbit polyclonal antibody (Product # 40-2300), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
40-2300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Chicken,
Dog,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Clone
ZMD.437
Immunogen
Synthetic peptide derived from the N-terminal region of human, dog, mouse, and rat ZO-1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with the ZO-1 protein. Based on sequence homology, reactivity with ZO-2 or ZO-3 is not expected. 40-2300 has been successfully used in immunofluorescence and immunohistochemistry. However, for western blotting we recommend the use of monoclonal anti-ZO-1 antibody (Cat. No. 339100), or other polyclonal ZO-1 antibodies (Cat. Nos. 617300 or 402200).
Reactivity has been confirmed with MDCK-II, Caco-2, Rat-1, and NRK52E cells by immunofluorescence. Reactivity has also been confirmed with frozen mouse liver, blood vessel (in heart), and mossy fiber terminal (in brain) tissues by immunohistochemistry and immunofluorescence, and with formalin-fixed, paraffin-embedded human pancreas, prostate, and small intestine tissues by immunohistochemistry. This antibody (or Cat. No. 187430) is recommended for immunohistochemical detection of ZO-1.
For immunohistochemistry in formalin-fixed, paraffin-embedded tissues, tissue pretreatment with pepsin is required prior to staining. Some lots of this antibody have successfully been used in western blotting using human, dog and rat cell lysates. For immunofluorescence methanol fixation is preferred even though paraformaldehyde fixation will also work.
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; unnamed protein product; zona occludens 1; zona occludens protein 1; zonula occludens 1 protein; zonula occludens protein 1
Gene Aliases: ZO-1; ZO1
UniProt ID: (Human) Q07157, (Dog) O97758, (Mouse) P39447, (Rat) A0A0G2K2P5
Entrez Gene ID: (Human) 7082, (Dog) 403752, (Mouse) 21872, (Rat) 292994
cell-cell junction assembly
positive regulation of cell proliferation
actin cytoskeleton organization
positive regulation of cell migration
actomyosin structure organization
adherens junction maintenance
maintenance of blood-brain barrier
ameloblast differentiation
negative regulation of apoptotic process
cell-cell junction organization
regulation of cytoskeleton organization
negative regulation of stress fiber assembly
protein localization to adherens junction
establishment of endothelial intestinal barrier
cell-cell adhesion
protein localization to cell-cell junction
regulation of cell junction assembly
protein localization to bicellular tight junction
positive regulation of sprouting angiogenesis
positive regulation of maintenance of permeability of blood-brain barrier
positive regulation of cell-cell adhesion mediated by cadherin
regulation of bicellular tight junction assembly
bicellular tight junction assembly
cellular response to calcium ion
blastocyst formation
sensory perception of sound
response to xenobiotic stimulus
response to lipopolysaccharide
negative regulation of vascular permeability
response to ethanol
response to magnetism
cellular response to glucose stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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