TaqMan™ GAPDH Control Reagents (human)
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Applied Biosystems™

TaqMan™ GAPDH Control Reagents (human)

The Applied Biosystems™ TaqMan™ GAPDH Control Reagents kit provides components for using human GAPDH as a normalization control in your詳細を見る
製品番号(カタログ番号)数量
402869100 reactions
製品番号(カタログ番号) 402869
価格(JPY)
64,200
Each
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数量:
100 reactions
The Applied Biosystems™ TaqMan™ GAPDH Control Reagents kit provides components for using human GAPDH as a normalization control in your real-time PCR reactions. The kit includes human control RNA, GAPDH TaqMan™ probe (JOE™ dye-labeled probe), and GAPDH primers. You can use either DNA or RNA as a target template.

• Pre-designed primers and JOE™ dye-labeled TaqMan™ probe eliminate assay design

• Rapid assay development guidelines can minimize optimization time

• Separate components provide flexibility in assay set-up

Comprehensive Guidelines Included
Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In
All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Notes on Use
The TaqMan™ GAPDH Control Reagents can be used with TaqMan™ Universal PCR Master Mix or TaqMan™ PCR Core Reagents for polymerase chain reaction (PCR).The TaqMan™ GAPDH Control Reagents can be used with the TaqMan™ One-Step RT-PCR Master Mix Reagents, TaqMan™ EZ RT-PCR Core Reagents, or the TaqMan™ Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).

The PCR process and 5' nuclease process are covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche, Ltd.

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
コントロールテンプレートHuman Control RNA
フォーマットTube
遺伝子記号GADPH
内部プローブの変更TAMRA™ Quencher (3'), JOE (5')
反応数反応100回分
製品ラインTaqMan
製品タイプReal Time PCR Reagents
数量100 reactions
出荷条件ドライアイス
Human
対応可能対象100反応
ターゲットGAPDH
GC-Rich PCR Performance
標識または色素JOE
PCR法1-step RT-qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
Control Primer-Probe Set(s) & Template(s)

よくあるご質問(FAQ)

ヒトのGAPDHキットはgDNAを検出しますか?

GAPDH検出に対応したキットのgDNA検出の有無については下記の通りです。

- Taqman® GAPDH Control Reagents, human, 402869, JOE®:gDNAを検出しません。
- Taqman® Endogenous Control, GAPDH for human, 4326317E, VIC® :gDNAを検出しません。
- Taqman® Endogenous Control, GAPDH, human, 4310884E, VIC®:gDNAを検出しません。
- Taqman® Endogenous Control, GAPDH, human, 4333764F, FAM:gDNAを検出しません。
- Taqman® Endogenous Control, GAPDH, human, 4333764T, FAM:gDNAを検出しません。
- Taqman® Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: gDNAを検出しません。

TaqMan® GAPDH Control REagents Kit(Cat. No. 402869)を利用した際のPCR増幅産物のサイズを知りたい。

増幅産物鎖長は226ベースです。設計はNCBI GenBank®のaccession number J04038の情報を利用しています。

In the TaqMan GAPDH Control Reagents Kit (Cat. No. 402869), what is the size of the GAPDH amplicon?

The amplicon length is 226 bases, and is based on the NCBI GenBank accession number J04038. More information on the GAPDH amplicon can be found in Appendix B of the “TaqMan Gold RT-PCR Kit” protocol that is found on the Product Detail Page for Cat. No. 402869. Enter the catalog number in the main Search field on our web site to view this page and the Protocol.

Do the human and rodent GAPDH primers and TaqMan probe sequences manufactured by Applied Biosystems detect genomic DNA?

This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products:

- TaqMan GAPDH Control Reagents, human, 402869, JOE: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH for human, 4326317E, VIC : Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4310884E, VIC: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764F, FAM: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764T, FAM: Does not detect genomic DNA
- TaqMan Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: Does not detect genomic DNA

Do your human and rodent GAPDH primers and TaqMan probe sequences detect genomic DNA?

It varies by product, so be sure to check all the available product specifications before purchase. Here are details on a few options:

- The TaqMan GAPDH Control Reagents (Human), Cat. No. 402869, which is labeled with JOE dye, spans two exons therefore will not amplify genomic DNA.

- The Human GAPD (GAPDH) Endogenous Control (VIC/ TAMRA Probe, Primer Limited), Cat. No. 4310884E, is also designed across an exon-exon boundary and will not amplify genomic DNA.

- The probe in the TaqMan Rodent GAPDH Control Reagents kit, Cat. No. 4308313, labeled with VIC dye, does not span an intron and therefore may amplify genomic DNA.