ヒトのGAPDHキットはgDNAを検出しますか?
GAPDH検出に対応したキットのgDNA検出の有無については下記の通りです。
- Taqman® GAPDH Control Reagents, human, 402869, JOE®:gDNAを検出しません。
- Taqman® Endogenous Control, GAPDH for human, 4326317E, VIC® :gDNAを検出しません。
- Taqman® Endogenous Control, GAPDH, human, 4310884E, VIC®:gDNAを検出しません。
- Taqman® Endogenous Control, GAPDH, human, 4333764F, FAM:gDNAを検出しません。
- Taqman® Endogenous Control, GAPDH, human, 4333764T, FAM:gDNAを検出しません。
- Taqman® Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: gDNAを検出しません。
TaqMan® GAPDH Control REagents Kit(Cat. No. 402869)を利用した際のPCR増幅産物のサイズを知りたい。
増幅産物鎖長は226ベースです。設計はNCBI GenBank®のaccession number J04038の情報を利用しています。
In the TaqMan GAPDH Control Reagents Kit (Cat. No. 402869), what is the size of the GAPDH amplicon?
The amplicon length is 226 bases, and is based on the NCBI GenBank accession number J04038. More information on the GAPDH amplicon can be found in Appendix B of the TaqMan Gold RT-PCR Kit protocol that is found on the Product Detail Page for Cat. No. 402869. Enter the catalog number in the main Search field on our web site to view this page and the Protocol.
Do the human and rodent GAPDH primers and TaqMan probe sequences manufactured by Applied Biosystems detect genomic DNA?
This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products:
- TaqMan GAPDH Control Reagents, human, 402869, JOE: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH for human, 4326317E, VIC : Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4310884E, VIC: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764F, FAM: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764T, FAM: Does not detect genomic DNA
- TaqMan Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: Does not detect genomic DNA
Do your human and rodent GAPDH primers and TaqMan probe sequences detect genomic DNA?
It varies by product, so be sure to check all the available product specifications before purchase. Here are details on a few options:
- The TaqMan GAPDH Control Reagents (Human), Cat. No. 402869, which is labeled with JOE dye, spans two exons therefore will not amplify genomic DNA.
- The Human GAPD (GAPDH) Endogenous Control (VIC/ TAMRA Probe, Primer Limited), Cat. No. 4310884E, is also designed across an exon-exon boundary and will not amplify genomic DNA.
- The probe in the TaqMan Rodent GAPDH Control Reagents kit, Cat. No. 4308313, labeled with VIC dye, does not span an intron and therefore may amplify genomic DNA.