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Rat anti-Mouse IgG2a Secondary Antibody, eFluor™ 570, eBioscience™
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FIGURE: 1 / 1
Mouse IgG2a Secondary Antibody (41-4210-82) in ICC/IF
Immunocytochemistry of fixed and permeabilized MDCK cells using either 5 µg/mL Mouse IgG2a Isotype Control (left) or 5 µg/mL Anti-Human PCNA Purified (Product # 14-9910-82) (right), followed by 5 µg/mL Anti-Mouse IgG2a eFluor® 570. Nuclei are stained with DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
41-4210-82
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
5 µg/mL
Immunohistochemistry (Frozen) (IHC (F))
5 µg/mL
Immunocytochemistry (ICC/IF)
5 µg/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Secondary Antibody
Clone
m2a-15F8
Conjugate
eFluor™ 570
eFluor™ 570
eFluor™ 570
Excitation/Emission Max
556/569 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG2a
Antibody Form
Whole Antibody
Product Specific Information
Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies.
Applications Reported: This m2a-15F8 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This m2a-15F8 antibody has been tested immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG2a isotype. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.
Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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