Interferon alpha Human ELISA Kit

Catalog number:  411001

 Related applications: Protein Assays and Analysis

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The Thermo Scientific Human Interferon alpha ELISA Kit (Supernatant) contains sufficient reagent to measure IFN-α levels in human cell culture media. This VeriKine* Human IFN alpha ELISA Kit (Supernatant) contains one precoated 96-well plate and sufficient solutions to generate a standard curve, including replicates, and assay several culture supernatants.

Features of the Human alpha ELISA Kit (Supernatant) IFN-&alpha:

Target: human interferon alpha (IFN-alpha)
Format: colorimetric ELISA (TMB substrate) in clear 96-well plate
Measurement: absorbance at 450nm
Assay Range: 12.5 to 500 pg/mL (high sensitivity); 156 to 5000 pg/mL (extended range)
Sensitivity: undetermined
Precision: intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
Sample Types: cell culture supernatant, tissue culture media
Total Assay Time: 3.25 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with human IFN-β, IFN-γ, or IFN-ω mouse or rat IFN-α, IFN-β, or IFN-γ bovine IFN-τ.

These human IFN alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 100 µL per well. These IFN-α kits are validated for use with human cell and tissue culture media and are optimized for both high-sensitivity and extended assay ranges. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: IFL, IFN, IFN-ALPHA, IFN-alphaD3, IFNA@, IFNA1, IFN-alphaA, IFNAB, INFA2, IFNA2
Gene ID: 3439, 3440
Gene Name: interferon alpha 1, interferon alpha 2
Gene Symbol: IFNA1, IFNA2
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFN-alpha-2, Interferon alpha-A, LeIF A
Protein Name: Interferon alpha-1/13, Interferon alpha-2
Target (Kit Named): Interferon alpha
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P01562, P01563

Contents & storage

Sufficient For: 96 microplate wells
• Precoated 96-well Plate, 1 each
• Plate Sealers, 4 ea.
• Wash Solution Concentrate, 50 mL
• Human IFN Alpha Standard 10,000 pg/mL, 0.5 mL
• Dilution Buffer, 50 mL
• Antibody Concentrate, 1 vial
• HRP Conjugate Concentrate, 1 vial
• HRP Conjugate Diluent, 15 mL
• TMB Substrate Solution, 15 mL
• Stop Solution, 15 mL


Manuals & protocols