Interferon alpha Human ELISA Kit

Catalog number:  411101

 Related applications: Protein Assays and Analysis

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The Thermo Scientific IFN-α (human interferon alpha) ELISA Kit is configured to measure Interferon alpha levels in human serum, plasma and tissue culture media. This VeriKine* Human IFN alpha ELISA Kit (for Serum Samples) contains one precoated 96-well plate, detection antibody, buffers, diluents, standards, substrate, and stop solutions.

Features of the Human IFN alpha ELISA Kit (Serum Samples):

Target: human interferon alpha (human IFN-α, IFN-alpha)
Format: colorimetric ELISA (TMB substrate) in clear 96-well plate
Measurement: absorbance at 450nm
Assay Range: 12.5 to 1000 pg/mL
Sensitivity: undetermined
Precision: intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
Sample Types: cell culture supernatant, serum, plasma
Total Assay Time: 3.25 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IFN-β, IFN-γ, or IFN-ω mouse or rat IFN-α, IFN-β, or IFN-γ bovine IFN-τ.

These human IFN alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 50 µL per well. These IFN-α kits are validated for use with human serum, plasma and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: IFL, IFN, IFN-ALPHA, IFN-alphaD3, IFNA@, IFNA1, IFN-alphaA, IFNAB, INFA2, IFNA2
Gene ID: 3439, 3440
Gene Name: interferon alpha 1, interferon alpha 2
Gene Symbol: IFNA1, IFNA2
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFN-alpha-2, Interferon alpha-A, LeIF A
Protein Name: Interferon alpha-1/13, Interferon alpha-2
Target (Kit Named): Interferon alpha
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P01562, P01563

Contents & storage

Sufficient For: 96 microplate wells
• Precoated 96-well Plate, 1 each
• Plate Sealers, 4 ea.
• Wash Solution Concentrate, 50 mL
• Human IFN Alpha Standard 10,000 pg/mL, 1 vial
• Sample Diluent, 30 mL
• Antibody Concentrate, 1 vial
• HRP Conjugate Concentrate, 1 vial
• Concentrate Diluent, 15 mL
• TMB Substrate Solution, 15 mL
• Stop Solution, 15 mL
• Dilution Buffer, 30 mL


Manuals & protocols