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Invitrogen
SMAD7 Polyclonal Antibody
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FIGURE: 1 / 16
SMAD7 Antibody (42-0400) in ICC/IF
Immunofluorescence analysis of SMAD7 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SMAD7 Rabbit Polyclonal Antibody (Product # 42-0400) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
42-0400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the C-terminal region of the human SMAD7
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ16482; hSMAD7; MAD (mothers against decapentaplegic, Drosophila) homolog 7; Mad homolog; MAD homolog 7; MAD homolog 8; madh8; mothers against decapentaplegic homolog 7; mothers against decapentaplegic homolog 8; Mothers against decapentaplegic, drosophila, homolog of, 7; mothers against DPP homolog 7; mothers against DPP homolog 8; SMAD; SMAD 7; SMAD, mothers against DPP homolog 7; unnamed protein product
Gene Aliases: CRCS3; MADH7; MADH8
UniProt ID: (Human) O15105, (Mouse) O35253, (Rat) O88406
Entrez Gene ID: (Human) 4092, (Mouse) 17131, (Rat) 81516
transcription corepressor activity
protein binding
collagen binding
beta-catenin binding
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
I-SMAD binding
activin receptor binding
transcription regulator inhibitor activity
protein binding, bridging involved in substrate recognition for ubiquitination
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
negative regulation of T cell cytokine production
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway
anatomical structure morphogenesis
regulation of epithelial to mesenchymal transition
regulation of transforming growth factor beta receptor signaling pathway
cell differentiation
negative regulation of ossification
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
protein complex localization
negative regulation of activin receptor signaling pathway
protein-containing complex disassembly
adherens junction assembly
response to laminar fluid shear stress
intracellular signal transduction
artery morphogenesis
protein stabilization
ventricular cardiac muscle tissue morphogenesis
regulation of cardiac muscle contraction
regulation of ventricular cardiac muscle cell membrane depolarization
negative regulation of SMAD protein import into nucleus
SMAD protein signal transduction
ventricular septum morphogenesis
negative regulation of chondrocyte proliferation
positive regulation of chondrocyte hypertrophy
beta-catenin destruction complex disassembly
cellular response to leukemia inhibitory factor
positive regulation of cell-cell adhesion mediated by cadherin
negative regulation of T-helper 17 type immune response
negative regulation of T-helper 17 cell differentiation
negative regulation of transcription, DNA-templated
negative regulation of epithelial to mesenchymal transition
negative regulation of cell migration
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
negative regulation of cell differentiation
cellular response to growth factor stimulus
transforming growth factor beta receptor superfamily signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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