To purchase this product online, please order this equivalent product, IFN-α Mouse ELISA Kit.

IFN alpha Mouse ELISA Kit

Catalog number:  421201

 Related applications: Protein Assays and Analysis

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Assay IFN-α (mouse interferon alpha) levels in mouse cell and tissue culture media (supernatants) with this VeriKine* Mouse IFN alpha ELISA Kit (for supernatant).

Features of the VeriKine* Mouse IFN alpha ELISA Kit:

Target: mouse interferon alpha (mouse IFN-α, IFN-alpha)
Format: colorimetric ELISA (TMB substrate) in clear 96-well plate
Measurement: absorbance at 450nm
Assay Range: 12.5 to 400 pg/mL
Sensitivity: undetermined
Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, tissue culture media
Total Assay Time: 27.25 hours (overnight)
Sample Size: 100 µL
Specificity: no significant cross-reaction with mouse IFN-β, IFN-γ human or rat IFN-α.

This mouse IFN alpha ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 100 µL per well. The IFN-α kit is validated for use with mouse cell and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, diluents, standards, substrate, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: Ifa1
Gene ID: 15962
Gene Name: interferon alpha 1
Gene Symbol: IFNA1
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: IFN-alpha-1, Interferon alpha family gene 1
Protein Name: Interferon alpha-1
Target (Kit Named): IFN alpha
Target Species (Named): Mouse
Target Species (Validated): Mouse
UniProt ID: P01572

Contents & storage

Sufficient For: 96 microplate wells
• Precoated 96-well Plate, 1 each
• Plate Sealers, 4 ea.
• Wash Solution Concentrate, 50 mL
• Mouse IFN Alpha Standard 10,000 pg/mL, 1 vial
• Sample Buffer, 50 mL
• Antibody Concentrate, 1 vial
• Concentrate Diluent, 15 mL
• HRP Conjugate Concentrate, 1 vial
• Assay Diluent, 15 mL
• TMB Substrate Solution, 15 mL
• Stop Solution, 15 mL


Manuals & protocols