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Invitrogen
alpha Actinin 4 Polyclonal Antibody
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FIGURE: 1 / 9
alpha Actinin 4 Antibody (42-1400) in ICC/IF
Immunofluorescence analysis of alpha Actinin 4 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Actinin 4 Rabbit Polyclonal Antibody (Product # 42-1400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at ... View More
Product Details
42-1400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the N-terminal region of the human, mouse, and orangutan alpha-Actinin-4 protein
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: actinin-Alpha-4; alpha actinin 4; Alpha-actinin-4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; Non-muscle alpha-actinin 4; transcriptional coactivator; unnamed protein product
Gene Aliases: ACTININ-4; ACTN4; C77391; FSGS; FSGS1
UniProt ID: (Human) O43707, (Mouse) P57780, (Rat) Q9QXQ0
Entrez Gene ID: (Human) 81, (Mouse) 60595, (Rat) 63836
RNA polymerase II regulatory region sequence-specific DNA binding
nucleoside binding
transcription coactivator activity
RNA binding
actin binding
integrin binding
calcium ion binding
protein binding
ligand-dependent nuclear receptor binding
chromatin DNA binding
identical protein binding
protein homodimerization activity
retinoic acid receptor binding
ion channel binding
metal ion binding
actin filament binding
ligand-dependent nuclear receptor transcription coactivator activity
protein complex binding
nuclear hormone receptor binding
poly(A) RNA binding
protein N-terminus binding
transport
protein transport
peroxisome proliferator activated receptor signaling pathway
regulation of apoptotic process
retinoic acid receptor signaling pathway
positive regulation of pinocytosis
actin filament bundle assembly
negative regulation of cellular component movement
positive regulation of cellular component movement
bicellular tight junction assembly
protein localization to bicellular tight junction
regulation of nucleic acid-templated transcription
response to hypoxia
actin cytoskeleton organization
vesicle transport along actin filament
positive regulation of cell migration
positive regulation of sodium:proton antiporter activity
tumor necrosis factor-mediated signaling pathway
positive regulation of transcription from RNA polymerase II promoter
muscle cell development
negative regulation of substrate adhesion-dependent cell spreading
positive regulation of NIK/NF-kappaB signaling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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