IFN beta ELISA Kit, Mouse

Catalog number: 424001

 Related applications: Protein Assays and Analysis

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Description

Assay IFN-β (mouse interferon beta) levels in mouse serum and culture media (supernatants) with this VeriKine* Mouse IFN beta ELISA Kit.

Features of the VeriKine* Mouse IFN beta ELISA Kit:

Target: mouse interferon beta (mouse IFN-β, IFN-beta)
Format: colorimetric ELISA (TMB substrate) in clear 96-well plate
Measurement: absorbance at 450nm
Assay Range: 15.6 to 1000 pg/mL
Sensitivity: undetermined
Precision: intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
Sample Types: serum, cell culture supernatant, tissue culture media
Total Assay Time: 3.25 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with human IFN-α, IFN-γ, IFN-κ, IFN-β Rat IFN-α, IFN-γ, IFN-β Mouse IFN-α, IFN-γ Feline IFN-α or Pig IFN-α.

This mouse IFN beta ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon beta (IFN-β) in samples at 100 µL per well. The IFN-β kit is validated for use with mouse cell and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, diluents, standards, substrate, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Mouse
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Sufficient For: 96 microplate wells
• Precoated 96-well Plate, 1 each
• Plate Sealers, 4 ea.
• Wash Solution Concentrate, 50 mL
• Mouse IFN Beta Standard 500,000 pg/mL, 50 µL
• Sample Diluent, 30 mL
• Antibody Concentrate, 1 vial
• HRP Conjugate Concentrate, 1 vial
• Concentrate Diluent, 30 mL
• TMB Substrate Solution, 15 mL
• Stop Solution, 15 mL

Documents

Manuals & protocols