TaqMan® Reverse Transcription Reagents

Catalog number: 4304134

Invitrogen™  Related applications: Reverse Transcription

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Description

TaqMan® Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. Advantages of TaqMan® Reverse Transcription Reagents:

• Contain MultiScribe® Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)16 included for flexibility
• Individual components provide versatility in assay set-up
• Compatible with two-step RT-PCR reactions

Using TaqMan® Reverse Transcription Reagents
TaqMan® Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reverse Transcriptase: MultiScribe™ MuLV
Downstream Application: Real-Time Quantitative PCR (qPCR)
Reaction Format: Separate Components
Sample Type (General): RNA
Product Size: 2,000 reactions
Final Product: cDNA (First-Strand)
Final Product Size(s): 5 kb or less
Optimal Reaction Temperature: 42° C

Contents & storage

Includes 10 units of Cat. No. N8080234. Each N8080234 kit contains:

• 1 × 100 µL of MultiScribe™ Reverse Transcriptase (50U/µL)
• 1 × 100 µL of RNase Inhibitor (20U/µL)
• 1 × 1 mL of dNTP Mixture (2.5 mM each dNTP)
• 1 × 100 µL of Oligo d(T)16 (50 µM)
• 1 × 100 µL of Random Hexamers (50 µM)
• 1 × 1.5 mL of 10X RT Buffer
• 1 × 1.5 mL of MgCl2 Solution

Reagents should be stored between -15°C and -25°C.

A note about the size: This kit contains enough reagents for 2000 reverse transcriptase reactions when the reactions are set up at 10 µL each.

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