TaqMan® Universal PCR Master Mix (individually packaged)

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Description

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

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Applied Biosystems® TaqMan® Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time.
• Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase
• Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instruments.
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• AmpErase® UNG protects against subsequent re-amplification from PCR products containing dU to minimize carry-over contamination
• Rapid assay development guidelines are provided to minimize optimization time
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, GeneAmp® 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold® DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: TaqMan™
Product Size: 10 x 5 mL
Sample Type (Specific): DNA (Genomic), cDNA

Contents & storage

Supplied at 2X concentration. The mix contains AmpliTaq Gold® DNA Polymerase, Uracil-DNA Glycosylase, dTNPs with dUTP, Passive Reference 1 and optimized buffer components. This pack contains 10 boxes with one 5 mL tube each, sufficient for 2000 reactions at the 50 uL total volume. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

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