The Applied Biosystems™ TaqMan™ Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells.
• Pre-designed primers and TaqMan™ probe eliminate assay design
• Rapid assay development guidelines can minimize optimization time
• Separate components provide flexibility in assay set-up
Comprehensive Guidelines Included
Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.
All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.
Notes on Use:
The TaqMan™ Rodent GAPDH Control Reagents can be used with TaqMan™ Universal PCR Master Mix or TaqMan™ PCR Core Reagents for polymerase chain reaction (PCR). The TaqMan™ Rodent GAPDH Control Reagents can be used with TaqMan™ One-Step RT-PCR Master Mix Reagents, TaqMan™ EZ RT-PCR Core Reagents, or the TaqMan™ Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).
See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.