TaqMan® Universal PCR Master Mix, no AmpErase® UNG

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Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time.
• Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase
• Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instruments.
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• Contains dUTP in the dNTP mix to protect against carry-over contamination, when used with AmpErase® UNG
• Rapid assay development guidelines are provided to minimize optimization time
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7300 System, StepOnePlus™, Standard Mode, GeneAmp® 9600, StepOne™, Standard Mode, 7000 System, 7700 System, 7900HT System, 7500 System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Polymerase: AmpliTaq Gold® DNA Polymerase
Product Size: 2,000 reactions
Shipping Condition: Wet Ice
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (Specific): cDNA, DNA (Genomic)
Primer-Probe Compatibility: TaqMan®

Contents & storage

Supplied at 2X concentration. The mix contains AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1 and optimized buffer components. This pack contains 10 X 5 mL tubes, sufficient for 2000 reactions at the 50 μL total volume. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols