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Invitrogen
EHMT2 Monoclonal Antibody (A8620A)
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FIGURE: 1 / 3
EHMT2 Antibody (435200) in ICC/IF
Immunofluorescent analysis of G9a monoclonal antibody (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a G9a mouse monoclonal antibody (Product # 435200) at a concnetration of 5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32723) at a dilution o... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
435200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
A8620A
Immunogen
Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with Baculovirus-expressed recombinant mouse G9a (15-114aa).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
physiological saline
Contains
0.1% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody specifically recognizes mouse G9a and cross reacts with human G9a.
Target Information
A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ankyrin repeat-containing protein; DKFZp686H08213; euchromatic histone-lysine N-methyltransferase 2; FLJ35547; G9A histone methyltransferase; H3 ly; H3-K9-Histone-Methyl-Transferase; H3-K9-HMTase 3; histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; HLA-B associated transcript 8; lysine N-methyltransferase 1C; NG36/G9a; protein G9a; unnamed protein product
Gene Aliases: BAT8; C6orf30; D17Ertd710e; G9A; GAT8; KMT1C; NG36
UniProt ID: (Human) Q96KQ7, (Mouse) Q9Z148
Entrez Gene ID: (Human) 10919, (Mouse) 110147
RNA polymerase II regulatory region sequence-specific DNA binding
transcription corepressor binding
p53 binding
protein binding
zinc ion binding
protein-lysine N-methyltransferase activity
histone methyltransferase activity
histone methyltransferase activity (H3-K9 specific)
histone methyltransferase activity (H3-K27 specific)
C2H2 zinc finger domain binding
H1-4K26 methyltransferase activity
histone H3K56 methyltransferase activity
histone H3 methyltransferase activity
histone H3K9 monomethyltransferase activity
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
regulation of DNA replication
methylation-dependent chromatin silencing
regulation of transcription from RNA polymerase II promoter
protein methylation
synaptonemal complex assembly
germ cell development
spermatid development
long-term memory
cellular response to starvation
fertilization
gene expression
peptidyl-lysine dimethylation
chromosome condensation
regulation of protein modification process
organ growth
phenotypic switching
regulation of gene expression, epigenetic
hypermethylation of CpG island
negative regulation of transcription, DNA-templated
behavioral response to cocaine
neuron fate specification
cellular response to cocaine
negative regulation of autophagosome assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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