Applied Biosystems™

TaqMan™ Reagents Starter Kit

Catalog number:  4352405

 Related applications: Real Time PCR (qPCR)

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The TaqMan® PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan® 5' nuclease assay with the TaqMan® PCR Master Mix. Gene-specific, ready to use TaqMan® Gene Expression Assays and Universal Master Mix provide you with a fast, simple way for measuring changes in gene expression.

• Simple procedure gives you quick results
• Hot-Start DNA Polymerase enzyme system minimizes nonspecific product amplification for reliable results

Ready-to-Use Gene Expression Solution
The TaqMan® Reagents Starter Kit contains a sample of TaqMan® Universal PCR Master Mix, and TaqMan® Gene-Expression Assay (Eukaryotic 18S rRNA), plus a sample of human Raji cDNA - everything you need for 18S gene-expression analysis. Simply combine these reagents according to the instructions to generate reproducible and quantitative gene-expression data.

A Complete, Integrated Gene-Expression System
Eliminate time-consuming master mix formulation and assay optimization with the TaqMan® Gene-Expression Assays and the TaqMan® Universal PCR Master Mix, by using universal cycling conditions and pre-optimized reagents.

Used With
7500 Real-Time PCR System with Dell™ Notebook, 7500 Real-Time PCR System with Dell™Tower, 7900HT Fast Real-Time PCR System with 384-Well Block Module, 7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory, 7900HT Fast Real-Time PCR System with Standard 96-Well Block Module, 7900HT System Automation Accessory Upgrade, 7900HT System Standard 384-Well Block Upgrade Kit, 7900HT System Standard 96-Well Block Upgrade Kit, ABI PRISM® 7000 Sequence Detection System (Notebook).

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7000 System, 7500 System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Hot Start: Built-In Hot Start
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: TaqMan™
Product Size: 1 kit
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Dry Ice
Target Gene: 18S rRNA
Target Specificity: Target-Specific

Contents & storage

This kit contains TaqMan® Universal PCR Master Mix, No AmpErase® UNG, Eukaryotic 18S rRNA TaqMan® Assay (with two unlabeled PCR primers and a labeled TaqMan® probe), plus a sample of human Raji cDNA.

Store the PCR mix at 2 - 8°C. Store the rest of the kit contents at -15 to -25°C.


Manuals & protocols