SYBR® Green PCR Master Mix

Catalog number: 4364346

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

• Premixed components stored at 2–8°C significantly reduce assay setup time
• SYBR® Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience
Applied Biosystems® SYBR® Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan® probes are required. SYBR® Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold® DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR® Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: SYBR
For Use With (Equipment): 7300 System, 7000 System, 7700 System, 7900HT System, StepOne™, Standard Mode, QuantStudio™, ViiA™ 7 System, StepOnePlus™, Standard Mode, 7500 System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: SYBR® Green
Product Size: 5 x 5 mL
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (Specific): cDNA, DNA (Genomic)
Primer-Probe Compatibility: Unlabeled Oligos

Contents & storage

The 5 × 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 1000 reactions based on a 50 µl reaction volume.

Store at 2–8°C.


Manuals & protocols

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