TaqMan® MicroRNA Reverse Transcription Kit

Catalog number: 4366597

Applied Biosystems™  Related applications: miRNA & Non-Coding RNA Analysis

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Description

The Applied Biosystems® TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for 1000 reactions of optimal performance in TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reverse Transcriptase: MultiScribe™ MuLV
Downstream Application: Real-Time Quantitative PCR (qPCR)
Reaction Format: Separate Components
Sample Type (General): RNA
Final Product: cDNA
Final Product Size(s): 200 bp or less
Optimal Reaction Temperature: 42° C
Product Size: 1,000 reactions
Shipping Condition: Dry Ice

Contents & storage

Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/µL), and MultiScribe™ RT enzyme (50U/µL) for 1000 reactions. Store at -15 to -25°C.

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