• Find Real-Time and Digital PCR technical tips, troubleshooting help, and resources within our new Real-Time PCR and Digital PCR Applications Support Center.
View current Real-Time PCR promotions for potential cost savings.
Applied Biosystems™

Power SYBR™ Green PCR Master Mix

Catalog number:  4367659

 Related applications:

Real Time PCR (qPCR)

| Real Time PCR-Based Gene Expression Profiling

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

• Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR
• The optimized formulation contains highly purified AmpliTaq Gold® DNA Polymerase, LD to offer greater sensitivity than classic SYBR® Green PCR master mix
Power SYBR® Green PCR Master Mix contains a blend of dTTP/dUTP that is compatible with AmpErase® UNG, to minimize carryover contamination
• Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations to minimize well-to-well variability that result from a variety of causes, such as pipetting error and sample evaporation
Power SYBR® Green reagent-based PCR chemistry easily replaces the existing SYBR® Green PCR Master Mix in Applied Biosystems® protocols using the same setup and thermal cycling conditions

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Power SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): 7300 System, 7500 System, 7700 System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Label or Dye: SYBR® Green
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Primer-Probe Compatibility: Unlabeled Oligos
Product Line: SYBR®
Product Size: 1 x 5 mL
Purity or Quality Grade: LD (Low DNA)
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Wet Ice

Contents & storage

The 2X mix contains SYBR® Green 1 Dye, AmpliTaq Gold DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Contains 1 × 5 mL, sufficient for 200 reactions of 50 µl each.

Store at -20°C.

Documents

Manuals & protocols