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Applied Biosystems™

High-Capacity cDNA Reverse Transcription Kit

Catalog number: 4368814
Applied Biosystems™

High-Capacity cDNA Reverse Transcription Kit

Catalog number: 4368814

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction. Both types of kit deliver extremely high-quality, single-stranded cDNA from 0.02–2 μg total RNA. Reactions can be scaled up to 100 μL to generate 10 μg of cDNA from a single reaction.

 
Catalog Number
4368814
Unit Size
Each
Includes
Kit only
No. of Reactions
200 Reactions
Price (USD)
Availability
-
Full specifications
Quantity
FormatKit
Reaction Speed120 min.
TechniqueReverse Transcription
Optimal Reaction Temperature37°C
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)RT-PCR
Concentration50 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)7 kb or Less
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit only
No. of Reactions
1,000 Reactions
Price (USD)
Availability
-
Full specifications
Quantity
FormatKit
Reaction Speed120 min.
TechniqueReverse Transcription
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Concentration50 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions1,000 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)7 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit with Rnase Inhibitor
No. of Reactions
200 Reactions
Price (USD)
Availability
-
Full specifications
Quantity
FormatKit
Reaction Speed120 min.
TechniqueReverse Transcription
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Concentration50 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)7 kb
Starting MaterialRNA
IncludesKit with Rnase Inhibitor
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit with Rnase Inhibitor
No. of Reactions
1,000 Reactions
Price (USD)
Availability
-
Full specifications
Quantity
FormatKit
Reaction Speed120 min.
TechniqueReverse Transcription
Optimal Reaction Temperature37°C
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)RT-PCR
Concentration50 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions1,000 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 7 kb
Starting MaterialRNA
IncludesKit with Rnase Inhibitor
Unit SizeEach
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeIncludesNo. of ReactionsPrice (USD)AvailabilityQuantity
4368814Full specifications
Each Kit only200 Reactions
-
FormatKit
Reaction Speed120 min.
TechniqueReverse Transcription
Optimal Reaction Temperature37°C
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)RT-PCR
Concentration50 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)7 kb or Less
Starting MaterialRNA
IncludesKit only
Unit SizeEach
4368813Full specifications
Each Kit only1,000 Reactions
-
FormatKit
Reaction Speed120 min.
TechniqueReverse Transcription
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Concentration50 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions1,000 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)7 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
4374966Full specifications
Each Kit with Rnase Inhibitor200 Reactions
-
FormatKit
Reaction Speed120 min.
TechniqueReverse Transcription
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Concentration50 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)7 kb
Starting MaterialRNA
IncludesKit with Rnase Inhibitor
Unit SizeEach
4374967Full specifications
Each Kit with Rnase Inhibitor1,000 Reactions
-
FormatKit
Reaction Speed120 min.
TechniqueReverse Transcription
Optimal Reaction Temperature37°C
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)RT-PCR
Concentration50 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions1,000 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 7 kb
Starting MaterialRNA
IncludesKit with Rnase Inhibitor
Unit SizeEach
Showing 4 of 4

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor includes the recombinant enzyme used to prevent degradation of RNA templates and lacks DNA endonuclease activity for better product yield.

In addition to their use in RT-PCR, this kit may be used in traditional PCR, microarrays, and for the generation of cDNA archives.

Features of the High Capacity cDNA Reverse Transcription Kit include:
• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. This kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

• 10X RT Buffer, 1 mL
• 10X RT Random Primers, 1 mL
• 25X dNTP Mix, 0.2 mL (100 mM)
• MultiScribe Reverse Transcriptase, 0.1 mL (50 U/μL)

Store at –15 to –25°C.
 

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