Applied Biosystems™

TaqMan™ Gene Expression Master Mix

Catalog number:  4369542

Related applications:

Industrial & Applied Science

| PCR & Real-Time PCR

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Description

Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan Gene Expression Master Mix has been validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications, including:
• Rare transcript detection
• Gene expression analysis
• Pathogen detection and viral load quantification
• Co-amplification of two targets
• Specific detection of gene family members

TaqMan Gene Expression Master Mix contains AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Gene Expression Master Mix and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity: 10 x 5mL
Detection Method: Primer-Probe Detection
Sample Type: DNA (Genomic), cDNA
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
PCR Method: qPCR
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Purity or Quality Grade: UP (Ultra Pure)
Primer Probe Compatibility: TaqMan®
Product Line: TaqMan™
Reaction Speed: Standard

Contents & storage

10 bottles (5 ml each) 2X TaqMan® Gene Expression Master Mix sufficient for 2000 reactions. Store at 2°C to 8°C.


TaqMan® Gene Expression Master Mix contains AmpliTaq Gold® DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX™ Passive Reference, and optimized buffer components.