TaqMan® Gene Expression Master Mix

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Get precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems® TaqMan® Gene Expression Master Mix. TaqMan® Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan® Gene Expression Master Mix has been validated with TaqMan® Gene Expression Assays and Applied Biosystems® real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications, including:
• Rare transcript detection
• Gene expression analysis
• Pathogen detection and viral load quantification
• Co-amplification of two targets
• Specific detection of gene family members TaqMan® Gene Expression Master Mix contains AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and Uracil-DNA Glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX™ dye for superb precision on Applied Biosystems® real-time PCR instruments.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7300 System, StepOnePlus™, Standard Mode, 7000 System, 7900HT System, 7500 System, StepOne™, Standard Mode
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Polymerase: AmpliTaq Gold® DNA Polymerase
Product Size: 1 x 50 mL
Shipping Condition: Wet Ice
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (Specific): cDNA, DNA (Genomic)
Purity or Quality Grade: UP (Ultra Pure)
Primer-Probe Compatibility: TaqMan®

Contents & storage

1 bottle (50 ml) 2X TaqMan® Gene Expression Master Mix sufficient for 2000 reactions. Store at 2°C to 8°C.

TaqMan® Gene Expression Master Mix contains AmpliTaq Gold® DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX™ Passive Reference, and optimized buffer components.


Manuals & protocols

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