High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, 200 reactions - FAQs

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can I order the RNase Inhibitor from the High-Capacity cDNA Reverse Transcription Kit separately?

Yes. You can order the RNase Inhibitor separately as Cat. No. N8080119.

Can one use the reagents from High Capacity RT kit to make cDNA for TaqMan MicroRNA Assay?

Yes. You can use the reagents from the High Capacity cDNA Kit PN 4368814 (without RNAse Inhibitor) or 4374966 (with RNAse Inhibitor). Make sure to use the reverse transcription primer provided from the TaqMan miRNA assay for the reverse transcription.

Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?

We recommend the following kits:

• High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
• High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).

Can I purchase the 10X RT Buffer from the High-Capacity cDNA Reverse Transcription Kit as a stand-alone item?

Yes, the 10X RT Buffer can be purchased as a stand-alone item (Cat. No. N8080010).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.