TaqMan® PreAmp Master Mix Kit

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Stretch your precious samples into more real-time PCR experiments with TaqMan® PreAmp Master Mix Kit. Applied Biosystems® TaqMan® PreAmp Master Mix Kit contains TaqMan® PreAmp Master Mix and TaqMan® Gene Expression Master Mix. The two work hand in hand to provide a seamless workflow for preamplification and quantitation of mRNA.

The TaqMan® PreAmp Master Mix Kit lets you:

• Amplify cDNA targets equally without introducing bias
• Analyze mRNA from any precious sample such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE)
• Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan® Gene Expression Assays
• Analyze up to 100 gene expression targets with minimal hands-on time

Preserves Equilibrium of Targets Without Pre-amplification Bias
In the past, preamplification kits and techniques often resulted in uneven amplification of targets and inaccurate or biased data (as shown by low correlation coefficients between amplified and unamplified samples). TaqMan® PreAmp Master Mix amplifies your small starting samples with no bias and provides extremely high correlation between amplified and unamplified cDNA.

Optimized Workflow and Results
TaqMan® PreAmp Master Mix and TaqMan® Gene Expression Master Mix work in conjunction to provide an optimal workflow for analysis of small starting samples. TaqMan® Gene Expression Master Mix provides reliable quantification over 9 logs of linear dynamic range and has been validated with TaqMan® Assays.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7300 System, 7500 Fast System, 7900HT Fast System, 7500 System
Reaction Speed: Standard, Fast
PCR Method: qPCR
Sample Type (General): DNA
Hot Start: Built-In Hot Start
Polymerase: AmpliTaq Gold® DNA Polymerase
Product Size: 40 reactions
Passive Reference Dye: No ROX
Sample Type (Specific): cDNA
Primer-Probe Compatibility: TaqMan®

Contents & storage

Contains 2X TaqMan PreAmp Master Mix (1 mL), TaqMan® Gene Expression Master Mix (5 mL), and TaqMan® PreAmp Master Mix Kit Quick Reference Card. Store master mixes at 2-8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols

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