TaqMan™ PreAmp Master Mix Kit - FAQs

There is a preamplification protocol included with our TaqMan Sample-to-SNP Kit. This is designed to work with a lysate sample.

The TaqMan PreAmp Master Mix Kit (P/N 4384267) is intended for use with very small quantities of cDNA (1 to 250 ng). This kit allows you to increase the quantity of specific cDNA targets for gene expression analysis using TaqMan Gene Expression Assays. Starting material is increased prior to PCR and the resulting preamplification product is then used for PCR. The TaqMan PreAmp Master Mix Kit enables multiplex preamplification of up to 100 targets at a time and provides unbiased amplification of specific amplicons for analysis with TaqMan Gene Expression Assays. For more detailed information on this kit, please refer to the TaqMan PreAmp Master Mix Kit Protocol (P/N 4384557).

The TaqMan PreAmp Master Mix Kit (P/N 4384267) preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your limited sample into many more real-time PCR reactions. TaqMan PreAmp Master Mix Kit consists of TaqMan PreAmp Master Mix and the TaqMan Gene Expression Master Mix. The two work hand in hand to provide the best results in preamplification and quantitation of mRNA.

For more detailed information on this kit, please refer to the TaqMan PreAmp Master Mix Kit Protocol (P/N 4384557).

TaqMan PreAmp Master Mix is designed for targeted preamplification of up to 100 targets from small amounts of cDNA, while SuperScript IV Single Cell/Low Input cDNA PreAmp Kit allows global preamplification of full-length cDNA from single cells or low input RNA. It provides reagents for cell lysis, reverse transcription and preamplification steps.