Fast SYBR® Green Master Mix

Catalog number: 4385618

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

Fast SYBR® Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR® Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq® Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR® Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOnePlus™, Fast Mode, StepOne™, Fast Mode
Reaction Speed: Fast
PCR Method: qPCR
Sample Type (General): DNA
Polymerase: AmpliTaq® Fast DNA Polymerase
Label or Dye: SYBR® Green
Product Size: 10 x 5 mL
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (Specific): cDNA, DNA (Genomic)
Purity or Quality Grade: UP (Ultra Pure)
Primer-Probe Compatibility: Unlabeled Oligos

Contents & storage

10 × 5 mL vials. The 2X mix contains SYBR® Green 1 Dye, AmpliTaq Gold® Fast DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1, and optimized buffer components. Sufficient for 5000 reactions of 20 µL each.

Store at 2–8°C.

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Manuals & protocols

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