High-Capacity RNA-to-cDNA™ Kit

Catalog number: 4387406

Applied Biosystems™  Related applications: Reverse Transcription

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Description

The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of this kit:

Fast—short reaction time (typically 30–60 min)
Convenient—easy workflow with few pipetting steps (2 tubes)
Reliable—reverse transcription of both abundant and limited targets
Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse transcription reaction

Streamline your cDNA synthesis while maintaining detection sensitivity
The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts. Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reverse Transcriptase: MultiScribe™ MuLV
Downstream Application: Real-Time Quantitative PCR (qPCR)
Reaction Format: SuperMix or Master Mix
Sample Type (General): RNA
Product Size: 50 reactions
Final Product: cDNA (First-Strand)
Shipping Condition: Dry Ice
Final Product Size(s): 7 kb or less
Optimal Reaction Temperature: 37° C

Contents & storage

Contents
• 1 × 50 µL of 20X Enzyme mix (MuLV and RNase inhibitor protein)
• 1 × 500 µL of 2X RT Buffer Mix (includes dNTPs, random octamers, and oligo dT-16)

Both components should be stored at -15°C to -25°C. 2X RT Buffer Mix can be stored at 2°C to 8°C for up to 6 months.

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Manuals & protocols

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