Applied Biosystems™

High-Capacity RNA-to-cDNA™ Kit

Catalog number: 4387406
Have Questions?
Applied Biosystems™

High-Capacity RNA-to-cDNA™ Kit

Catalog number: 4387406
Have Questions?
Catalog Number
4387406
Unit Size
50 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
438740650 reactions
-
4388950500 reactions
-
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The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts.

Features of the High Capacity RNA-to-cDNA Kit include:
• Fast reaction time (typically 30–60 min)
• Convenient and simple workflow with few pipetting steps
• Reliable reverse transcription of both abundant and limited targets
• Optimized two-step protocol enables multiple PCR reactions from a single RT reaction

Streamline cDNA synthesis while maintaining detection sensitivity
Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Reaction Speed
30 to 60 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
37°C
Reverse Transcriptase
MultiScribe
Ribonuclease H Activity
Yes
Shipping Condition
Dry Ice
For Use With (Application)
Real Time PCR (qPCR), RT-PCR
Final Product Type
First-Strand cDNA
No. of Reactions
50 Reactions
Reaction Format
Premixed Components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 7 kb
Starting Material
RNA

Contents & Storage

• 20X Enzyme mix (1 x 50 μL)
• 2X RT Buffer Mix (1 x 500 μL)

Store at –15 to –25°C.
Buffer Mix may be stored at 2–8°C for up to 6 months.

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