Informational:Informational:High-Capacity RNA-to-cDNA Kit now available in 500-reaction size. Learn more »
Applied Biosystems™

High-Capacity RNA-to-cDNA™ Kit

Catalog number: 4387406
Applied Biosystems™

High-Capacity RNA-to-cDNA™ Kit

Catalog number: 4387406
Catalog Number
4387406
Unit Size
50 reactions
Price (USD)
290.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
2,570.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
438740650 reactions
290.00
Your Price:
***
4388950500 reactions
2,570.00
Your Price:
***
Product Overview
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The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Features of this kit include:
Fast—short reaction time (typically 30–60 min)
Convenient—easy workflow with few pipetting steps
Reliable—reverse transcription of both abundant and limited targets
Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse transcription reaction

Streamline your cDNA synthesis while maintaining detection sensitivity
The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts. Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

Specifications

Detection Method
Primer-Probe
Final Product Type
cDNA (First-Strand)
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
50 Reactions
PCR Method
RT-PCR
Reaction Format
SuperMix or Master Mix
Reaction Speed
Fast
Sample Type
RNA
Size (Final Product)
7 kb or less
For Use With (Application)
Real-Time Quantitative PCR (qPCR)
No. of Tests
50 Reactions
Optimal Reaction Temperature
37° C
Product Line
TaqMan™
Product Type
High-Capacity RNA to cDNA Kit
Reverse Transcriptase
MultiScribe™
Shipping Condition
Dry Ice
Sufficient For
50 Reactions

Contents & Storage

Contents
• 1 × 50 µL of 20X Enzyme mix (MuLV and RNase inhibitor protein)
• 1 × 500 µL of 2X RT Buffer Mix (includes dNTPs, random octamers, and oligo dT-16)

Both components should be stored at -15°C to -25°C. 2X RT Buffer Mix can be stored at 2°C to 8°C for up to 6 months.

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