Applied Biosystems™

High-Capacity RNA-to-cDNA™ Kit

Catalog number: 4388950
Applied Biosystems™

High-Capacity RNA-to-cDNA™ Kit

Catalog number: 4388950
Catalog Number
4388950
Unit Size
500 reactions
Price (USD)
Price: 2,750.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
50 reactions
Price (USD)
Price: 310.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4388950500 reactions
Price: 2,750.00
Your Price:
***
438740650 reactions
Price: 310.00
Your Price:
***
Product Overview
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The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Features of this kit include:
Fast—short reaction time (typically 30–60 min)
Convenient—easy workflow with few pipetting steps
Reliable—reverse transcription of both abundant and limited targets
Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse transcription reaction

Streamline your cDNA synthesis while maintaining detection sensitivity
The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts. Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Real Time PCR (qPCR)
Detection Method
Primer-Probe
Final Product Type
cDNA (First-Strand)
Format
Kit
GC-Rich PCR Performance
High
Hot Start
No
No. of Reactions
500 Reactions
PCR Method
RT-PCR, RT-qPCR
Reaction Format
Master Mix
Reaction Speed
Standard
Reagent Type
Reverse Transcription
Sample Type
RNA
Size (Final Product)
7 kb or Less
Starting Material
RNA
Technique
Reverse Transcription
Volume
500 μL
Product Line
Applied Biosystems™
Product Type
High-Capacity RNA to cDNA Kit
Reverse Transcriptase
MultiScribe™ MuLV
Shipping Condition
Dry Ice

Contents & Storage

• 1 x 500 μL enzyme mix (MuLV and RNase inhibitor protein) (20X)
• 5 x 1 mL RT Buffer Mix (2X) (includes dNTPs, random octamers, and oligo dT-16)

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