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Invitrogen
NDUFS3 Monoclonal Antibody (17D95)
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FIGURE: 1 / 2
NDUFS3 Antibody (43-9200) in WB
Western blot analysis of NDUFS3 in isolated mitochondria and whole cell lysates using an NDUFS3 Monoclonal antibody (Product # 43-9200). The secondary antibody used was a Goat anti-Mouse IgG antibody. Lane 1: Isolated mitochondria from Human heart at 5 µg, Lane 2: Isolated mitochondria from Bovine heart at 4 µg, Lane 3: Isolated mitochondria from Rat heart at 10 µg, Lane 4: Isolated mitochondria from Mouse heart at 10 µg. Predicted band size: 30 kDa, Observed band size: 26 kDa. Extra bands in the mouse sample (lane 4) are due to the reacti... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
43-9200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
17D95
Immunogen
Bovine Heart Complex I
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: Human heart mitochondria. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HepG2 cells.
Target Information
This gene encodes one of the iron-sulfur protein components of mitochondrial NADH:ubiquinone oxidoreductase. Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CI-30kD; complex I 30kDa subunit; complex I iron sulphur protein subunit; 30 kDa subunit; complex I-30kD; NADH dehydrogenase (ubiquinone) Fe-S protein 3; NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase); NADH dehydrogenase-ubiquinone 30 kDa subunit; NADH-ubiquinone oxidoreductase 30 kDa subunit; unnamed protein product
Gene Aliases: 0610010M09Rik; CI-30; CI-30kD; MC1DN8
UniProt ID: (Bovine) P23709, (Human) O75489, (Mouse) Q9DCT2
Entrez Gene ID: (Bovine) 287327, (Human) 4722, (Rat) 295923, (Mouse) 68349
negative regulation of cell growth
oxidation-reduction process
reactive oxygen species metabolic process
negative regulation of intrinsic apoptotic signaling pathway
mitochondrial electron transport, NADH to ubiquinone
aerobic respiration
substantia nigra development
mitochondrial respiratory chain complex I assembly
mitochondrial ATP synthesis coupled proton transport
hydrogen ion transmembrane transport
biological_process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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