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TaqMan® Environmental Master Mix 2.0

Catalog number: 4398021

Applied Biosystems™  Related applications: Diagnostic Testing | Real Time PCR (qPCR)

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Description

Applied Biosystems® TaqMan® Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors. Use this Master Mix to analyze environmental, food and other challenging samples.

- Tailored for quantitative, real-time PCR experiments
- Unrivalled sensitivity for challenging applications, low copy number target detection and multiplex PCR
- Includes pathogen detection and viral⁄bacterial load quantification
- Sensitive detection for reliable quantification of abundant and limited genetic targets
- Stable mix for high-throughput handling

Optimized Formulation for Unrivalled Performance

TaqMan® Environmental Master Mix 2.0 is a convenient 2X mix for target quantification that includes:

- AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure), a highly purified DNA Polymerase for improved detection of bacterial targets. This hot-start enzyme is inactive at room temperature so reactions can be set up on the benchtop. Enzyme is activated during thermal cycling.
- Passive internal reference based on proprietary ROX™ dye for precise data analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
PCR Method: qPCR
For Use With (Equipment): 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type (General): Environmental Samples
Sample Type (Specific): Environmental Sample, Food
Hot Start: Built-In Hot Start
Passive Reference Dye: ROX (Pre-mixed)
Reaction Speed: Standard
Polymerase: AmpliTaq Gold® DNA Polymerase
Number of Reactions: 400 Reactions
Product Line: TaqMan™
Product Size: 400 reactions
Purity or Quality Grade: UP (Ultra Pure)

Contents & storage

TaqMan® Environmental Master Mix 2.0 is a convenient 2X mix in a 10mL bottle that includes:AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) and a passive internal reference based on proprietary ROX™ dye for precise data analysis.
Upon receipt, store at -15 to -25 °C before first use, protected from light. After first use, store at 2 to 8 °C, protected from light.

Documents

Manuals & protocols