Applied Biosystems® Megaplex™ Primer Pools, Rodent Pool A is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools A. Megaplex™ RT Primers, Rodent Pools A contains RT primers for 335 and 226 unique microRNAs for mouse and rat respectively plus 4 species specific controls. For use when assay sensitivity of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Rodent Pool B. Rodent Pool A is intended for use with the TaqMan® Rodent MicroRNA A Array v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR Performance
High
PCR Method
qPCR
Green Features
Sustainable packaging
Primer
RT Primers, PreAmp Primers
Product Line
Megaplex™, TaqMan™
Product Type
Primer Pool
Species
Rat, Mouse
Sufficient For
50 Reactions
For Use With (Application)
miRNA analysis
Concentration
10X
Fidelity (vs. Taq)
10 X
No. of Reactions
50 Reactions
Contents & Storage
Contains 150μL of 10X primers (Megaplex™ PreAmp Primers, Rodent Pool A) and 50μL of 10X primer (Megaplex™ RT Primers, Rodent Pool A). Store at -15 to -25°C.