TaqMan™ GTXpress™ Master Mix
TaqMan™ GTXpress™ Master Mix
TaqMan™ GTXpress™ Master Mix
TaqMan™ GTXpress™ Master Mix
Applied Biosystems™

TaqMan™ GTXpress™ Master Mix

**********************Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing genotyping master mix for accurate results, even fromRead more
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Catalog NumberQuantity
440189210 mL
44033111 mL
440189050 mL
44018572 x 50 mL
Catalog number 4401892
Price (MXN)
-
Quantity:
10 mL
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Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing genotyping master mix for accurate results, even from samples containing PCR inhibitors. With TaqPath ProAmp Master Mix, we’ve taken the best of TaqMan GTXpress Master Mix and added additional capabilities for your genotyping experiments.

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TaqMan™ GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems™ TaqMan™ GTXpress™ Master Mix (2X) combines AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 μL/reaction).
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
• Extended PCR cycling can be used for poorly amplified or sample-limited reactions The TaqMan™ GTXpress™ Master Mix is also sold as part of TaqMan™ Sample-to-SNP™ Kit, which provides real-time PCR-ready DNA in 5 min from a wide variety of samples, ranging from blood to buccal swabs to plant tissues. For more information on the TaqMan™ Sample-to-SNP™ Kit, refer to the Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit Protocol (PN. 4402136).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)2 X
For Use With (Equipment)QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™ Standard Mode, StepOne™ Fast Mode
FormatSingle Vial
Genotyping TargetSNPs (Unknown or Numerous), SNPs (Known)
Hot StartBuilt-In Hot Start
No. of Reactions40,000 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq DNA Polymerase
Product LineGTXpress, TaqMan
Product TypeMaster Mix
Purity or Quality GradeUP (Ultra Pure)
Quantity10 mL
Sample TypeDNA (Genomic), Sample-to-SNP™ Kit Lysates
Shipping ConditionWet Ice
TechniqueSNP Genotyping (Real-Time PCR-Based)
Volume100 mL
Concentration2X
Detection MethodPrimer-probe
For Use With (Application)Genotyping
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedFast or Standard
Unit SizeEach
Contents & Storage
Contains 1 x 10 mL TaqMan™ GTXpress™ Master Mix (2X mix of AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye and ROX™ Dye). Sufficient for 4,000 reactions. Store at 2-8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Frequently asked questions (FAQs)

What master mix can I use with the TaqMan SNP Genotyping Assays?

We recommend using either the TaqPath ProAmp Master Mix or the TaqMan Genotyping Master Mix. The TaqMan Genotyping Master Mix has the advantage of proven performance with up to 3 days of pre- and post-PCR stability, allowing you to set up plates ahead of time or read the plates later (see the data here, https://tools.thermofisher.com/content/sfs/brochures/cms_039236.pdf) while the TaqPath ProAmp Master Mix can handle samples that may have inhibitors present.

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.