VetMAX™-Plus One-Step RT-PCR Kit

For use with VetMAX™ Reagents and VetMAX™ Controls. The VetMAX™-Plus One-Step RT-PCR Kit is designed for robust amplification of animalMás información

Número de catálogo	Cantidad
4415328	100 reactions

Número de catálogo 4415328

Precio (MXN)

Cantidad:

100 reactions

For use with VetMAX™ Reagents and VetMAX™ Controls. The VetMAX™-Plus One-Step RT-PCR Kit is designed for robust amplification of animal pathogen RNA targets using a rapid, single-tube, real-time, reverse transcription PCR (RT-PCR) strategy. The kit includes Xeno™ RNA Control, which serves as an internal positive control for RNA purification and RT-PCR amplification. RNA is reverse-transcribed into cDNA, and then your target is amplified using AmpliTaq Gold® DNA Polymerase on a real-time PCR system.

Especificaciones

Para utilizar con (equipo)7500 Fast System, 7500 System

FormatoTubo

Inicio en calienteBuilt-In Hot Start

N.º de reacciones100 reacciones

Colorante de referencia pasivaROX (premezclado)

PolimerasaADN polimerasa AmpliTaq Gold

Línea de productosVetMAX

Tipo de productoPCR Reagents and Detection Kit for Animal Health

Cantidad100 reactions

Transcriptasa inversaArrayScript™ UP

Tipo de muestraARN

Condiciones de envíoDry Ice

Suficiente para100 reacciones

Método de detecciónSonda de cebado

Para utilizar con (aplicación)Gene Expression

FormularioLíquido

GC-Rich PCR PerformanceLow

Método de PCR1-step RT-qPCR

Velocidad de reacciónEstándar

Unit SizeEach

Contenido y almacenamiento

Store at -20°C. Includes 100 reactions with the following components: RT-PCR Buffer, RT-PCR Enzyme Mix, Xeno™ RNA Control, and Nuclease-free water.

Preguntas frecuentes

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all VetMAX™-Plus One-Step RT-PCR Kit FAQs