VetMAX™-Plus Multiplex One Step RT-PCR Kit
Applied Biosystems™

VetMAX™-Plus Multiplex One Step RT-PCR Kit

For use with VetMAX™ Reagents and VetMAX™ Controls. The VetMAX™-Plus Multiplex One-Step RT-PCR Kit is designed for multiplex amplification ofAfficher plus
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RéférenceQuantité
4415330100 reactions
Référence 4415330
Prix (EUR)
550,00
Each
Quantité:
100 reactions
Prix (EUR)
550,00
Each
For use with VetMAX™ Reagents and VetMAX™ Controls. The VetMAX™-Plus Multiplex One-Step RT-PCR Kit is designed for multiplex amplification of animal pathogen RNA targets using a rapid, single-tube, real-time, reverse transcription PCR (RT-PCR) strategy. The kit includes Xeno™ RNA Control, which serves as an internal positive control for RNA purification and RT-PCR amplification. RNA is reverse-transcribed into cDNA, and then your target is amplified using AmpliTaq Gold® DNA Polymerase on a real-time PCR system.
Spécifications
À utiliser avec (équipement)7500 Fast System, 7500 System
FormatTube
Hot StartBuilt-In Hot Start
Nbre de réactions100 réactions
Colorant de référence passiveROX (pré-mélangé)
PolyméraseAmpliTaq Gold DNA Polymerase
Gamme de produitsVetMAX
Type de produitPCR Reagents and Detection Kit for Animal Health
Quantité100 reactions
Transcriptase inverseArrayScript™ UP
Type d’échantillonARN
Conditions d’expéditionDry Ice
Suffisant pour100 réactions
Méthode de détectionAmorce-sonde
À utiliser avec (application)Gene Expression
FormeLiquide
GC-Rich PCR PerformanceLow
Méthode PCR1-step RT-qPCR
Vitesse de réactionÉtalon
Unit SizeEach
Contenu et stockage
Store at -20°C. Includes 100 reactions with the following components: Multiplex RT-PCR Buffer, Multiplex RT-PCR Enzyme Mix, Xeno™ RNA Control, and Nuclease-free water.

Foire aux questions (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.