Applied Biosystems™

Megaplex™ PreAmp Primers, Rodent Pool Set v3.0

Pour une utilisation lorsque la sensibilité du dosage est capitale, les amorces PreAmp Megaplex™ améliorent significativement la capacité de détecterAfficher plus

Référence	Quantité
4444747	50 réactions

Référence 4444747

Prix (EUR)

1 154,00

Quantité:

50 réactions

Prix (EUR)

1 154,00

Pour une utilisation lorsque la sensibilité du dosage est capitale, les amorces PreAmp Megaplex™ améliorent significativement la capacité de détecter les microARN à faible expression, permettant la génération d’un profil d’expression complet en utilisant beaucoup moins d’échantillons, dès 1 ng d’ARN total d’entrée.Avec une teneur correspondant aux amorces Megaplex™ RT et aux amorces Megaplex™ PreAmp, l’ensemble de groupes de rongeurs v3.0 est utilisé avec les amorces Megaplex™ RT, ensemble de groupes de rongeurs v3.0.Suite à une transcription inverse à l’aide d’amorces Megaplex™ RT, les amorces Megaplex™ PreAmp ainsi que le Master Mix TaqMan® PreAmp amplifient uniformément tous les micro-ARN avant la quantification par PCR en temps réel à l’aide de grilles de micro-ARN TaqMan®.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Nbre de réactions50 réactions

AmorceAmorces PreAmp

Gamme de produitsMegaplex, TaqMan

Type de produitAmorce de préamplification

Quantité50 réactions

EspècesRat, souris

Suffisant pour50 réactions

GC-Rich PCR PerformanceFaible

Méthode PCRqPCR

Unit SizeEach

Contenu et stockage

Stockage à -20°C.

Foire aux questions (FAQ)

Can Ct's greater than a cut-off be considered valid results?

Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

View all Megaplex™ PreAmp Primers, Rodent Pool Set v3.0 FAQs