Applied Biosystems™

resDNASEQ™ Quantitative MDCK DNA Kits

Catalog number: 4464335
Applied Biosystems™

resDNASEQ™ Quantitative MDCK DNA Kits

Catalog number: 4464335
The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ™ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples to final product, if the sample contains high molecular weight or sheared DNA.
 
Catalog Number
4464335
Unit Size
100 reactions
Includes
Assay Kit only
Price (USD)
Price: 3,420.00
Your price:
Availability
-
Full specifications
Quantity
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellMDCK
Label or DyeFAM
Product LineresDNASEQ™
Shipping ConditionDry Ice
No. of Reactions100 Reactions
IncludesAssay Kit only
Unit Size100 reactions
Catalog Number
Unit Size
100 reactions
Includes
Assay Kit and Sample Preparation
Price (USD)
Price: 4,130.00
Your price:
Availability
-
Full specifications
Quantity
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellMDCK
Label or DyeFAM
Product LineresDNASEQ™
No. of Reactions100 Reactions
IncludesAssay Kit and Sample Preparation
Unit Size100 reactions
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeIncludesPrice (USD)AvailabilityQuantity
4464335Full specifications
100 reactionsAssay Kit only
Price: 3,420.00
Your price:
-
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellMDCK
Label or DyeFAM
Product LineresDNASEQ™
Shipping ConditionDry Ice
No. of Reactions100 Reactions
IncludesAssay Kit only
Unit Size100 reactions
4464339Full specifications
100 reactionsAssay Kit and Sample Preparation
Price: 4,130.00
Your price:
-
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellMDCK
Label or DyeFAM
Product LineresDNASEQ™
No. of Reactions100 Reactions
IncludesAssay Kit and Sample Preparation
Unit Size100 reactions
Showing 2 of 2
Features of the resDNASEQ Quantitative MDCK DNA Kit include:
• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan™ primer/probe mix, and MDCK genomic DNA standard

This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.

The resDNASEQ™ Quantitative MDCK DNA Kit is designed to provide highly sensitive detection of MDCK DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan™ real-time qPCR technology allows you to test samples with variable levels of MDCK DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Easy to use
With the resDNASEQ™ Quantitative MDCK DNA Kit you perform qPCR analysis to compare the amount of MDCK DNA in your test samples to a standard curve generated with known amounts of purified MDCK genomic DNA standard. The resDNASEQ™ kit includes almost everything you need to carry out these steps, from master mix to TaqMan™ primer/probe mix to MDCK genomic DNA standard, as well as step-by-step instructions.

Specific to MDCK DNA, no cross-reactivity with unrelated DNA
The target of the assay is highly specific—it only detects a MDCK-specific region of a multicopy genetic element. We selected this region using extensive bioinformatic analysis of multiple related and unrelated species.

Consistent performance even with fragmented DNA
For accurate quantitation of residual MDCK DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ™ system you get consistent assay performance across a range of DNA fragment sizes.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

resDNASEQ MDCK DNA Control
• MDCK DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C
• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use

resDNASEQ MDCK DNA Real-Time PCR Reagents
• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• MDCK DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use

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