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          • Primary Antibodies ›
          • SRC Antibodies

          Invitrogen

          Phospho-SRC (Tyr529) Polyclonal Antibody

          Advanced Verification
          2 Published Figures
          14 References
          View all (72) SRC antibodies

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          Cite Phospho-SRC (Tyr529) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          • Published Figures (2)
          • Advanced Verification (1)
          Phospho-SRC (Tyr529) Antibody in Immunocytochemistry (ICC/IF)
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          Phospho-SRC (Tyr529) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          Phospho-SRC (Tyr529) Antibody (44-662G) in ICC/IF

          Immunofluorescence analysis of SRC (pY529) was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SRC (pY529) Rabbit Polyclonal Antibody (Product # 44-662G) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temp... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-SRC (Tyr529) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-SRC (Tyr529) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-SRC (Tyr529) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-SRC (Tyr529) Antibody in Western Blot (WB)
          Phospho-SRC (Tyr529) Antibody in Western Blot (WB)
          Phospho-SRC (Tyr529) Antibody in Western Blot (WB)
          Phospho-SRC (Tyr529) Antibody in Western Blot (WB)
          Phospho-SRC (Tyr529) Antibody
          Phospho-SRC (Tyr529) Polyclonal Antibody

          Product Details

          44-662G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 11 publications 11 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1 µg/mL
          -

          Immunoprecipitation (IP)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Chicken, Human, Mouse, Rat

          Published species

          Bovine, Dog, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 529 (tyrosine 530 including the initiating methionine). The sequence is conserved in mouse, rat, chicken and frog.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533715

          Target Information

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: c-Src; c-src protein, C terminus; EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; non-tyrosine protein kinase; ORF; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-scr; pp60c-src; pp60src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; Rous sarcoma oncogene; RP5-823N20.1; src downstream region; tyrosine kinase pp60c-src; tyrosine protein kinase c-src; tyrosine protein kinase pp60-c-src; tyrosine-protein kinase SRC-1; Unknown; unnamed protein product; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma viral oncogene

          View more View less

          Gene Aliases: ASV; AW259666; c-SRC; p60-Src; PP60C-SCR; pp60c-src; SDR; SRC; SRC1; THC6

          View more View less

          UniProt ID: (Chicken) P00523, (Human) P12931, (Mouse) P05480, (Rat) Q9WUD9

          View more View less

          Entrez Gene ID: (Chicken) 396442, (Human) 6714, (Mouse) 20779, (Rat) 83805

          View more View less

          Function(s)
          protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding protein phosphatase binding heme binding growth factor receptor binding connexin binding nucleotide binding protein kinase activity integrin binding phospholipase activator activity kinase activity transferase activity enzyme binding protein domain specific binding protein tyrosine kinase activator activity receptor activator activity ionotropic glutamate receptor binding SH2 domain binding phospholipase binding ion channel binding cadherin binding ephrin receptor binding ATPase binding phosphoprotein binding BMP receptor binding scaffold protein binding protein kinase C binding insulin receptor binding protein C-terminus binding transferase activity, transferring phosphorus-containing groups protein kinase binding estrogen receptor binding protein complex binding cell adhesion molecule binding hormone receptor binding transmembrane signal receptor scaffold/adaptor protein
          Process(es)
          cell cycle cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway cell differentiation osteoclast development peptidyl-tyrosine autophosphorylation regulation of cell proliferation innate immune response bone resorption progesterone receptor signaling pathway regulation of cell cycle negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway negative regulation of transcription from RNA polymerase II promoter stimulatory C-type lectin receptor signaling pathway neutrophil activation involved in immune response immune system process negative regulation of inflammatory response to antigenic stimulus signal transduction signal complex assembly transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of protein processing macroautophagy cell migration protein deubiquitination peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion platelet activation forebrain development T cell costimulation negative regulation of protein complex assembly protein destabilization cellular response to nutrient levels negative regulation of telomere maintenance regulation of intracellular estrogen receptor signaling pathway positive regulation of integrin activation toll-like receptor 3 signaling pathway regulation of toll-like receptor 3 signaling pathway substrate adhesion-dependent cell spreading cellular response to reactive oxygen species positive regulation of dephosphorylation negative regulation of hippo signaling intracellular signal transduction cellular response to platelet-derived growth factor stimulus Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway angiotensin-activated signaling pathway TORC1 signaling vasodilation odontogenesis negative regulation of apoptotic process regulation of vascular permeability stress fiber assembly proteasome-mediated ubiquitin-dependent protein catabolic process regulation of bone resorption positive regulation of Notch signaling pathway positive regulation of glycolytic process viral entry into host cell protein autophosphorylation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway focal adhesion assembly oogenesis leukocyte migration positive regulation of small GTPase mediated signal transduction negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly intestinal epithelial cell development interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of podosome assembly positive regulation of protein serine/threonine kinase activity regulation of heart rate by cardiac conduction positive regulation of canonical Wnt signaling pathway positive regulation of protein localization to nucleus negative regulation of neutrophil activation negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of lamellipodium morphogenesis positive regulation of platelet-derived growth factor receptor-beta signaling pathway regulation of early endosome to late endosome transport negative regulation of anoikis regulation of caveolin-mediated endocytosis protein phosphorylation cell proliferation positive regulation of gene expression positive regulation of glucose metabolic process positive regulation of smooth muscle cell migration phosphorylation peptidyl-serine phosphorylation positive regulation of protein autophosphorylation activation of protein kinase B activity negative regulation of protein homooligomerization adherens junction organization positive regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of protein binding positive regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity transcytosis positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell adhesion negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of insulin receptor signaling pathway neurotrophin TRK receptor signaling pathway positive regulation of cytokine secretion positive regulation of protein transport angiotensin-activated signaling pathway involved in heart process positive regulation of intracellular signal transduction positive regulation of DNA biosynthetic process central nervous system development response to mechanical stimulus response to virus response to acidic pH positive regulation of platelet-derived growth factor receptor signaling pathway single organismal cell-cell adhesion intracellular estrogen receptor signaling pathway response to nutrient levels cellular response to insulin stimulus response to drug response to hydrogen peroxide response to mineralocorticoid response to electrical stimulus response to fatty acid cellular response to lipopolysaccharide cellular response to fatty acid cellular response to hypoxia regulation of podosome assembly
          It has to be done as per old AB suggested Products section.

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