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Invitrogen
Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal Antibody
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FIGURE: 1 / 56
Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (44-682G) in ICC/IF
Immunofluorescent analysis of JNK1/2 (pT183/pT185) was done on 70% confluent log phase A549 cells treated with Anisomycin (25 µg/mL for 30 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with JNK1/2 (pT183/pT185) Rabbit polyclonal Antibody (Product # 44-68... View More
Product Details
44-682G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Dog,
Human,
Insect,
Mouse,
Non-human primate,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human JNK1&2 that contains threonine 183 and tyrosine 185. This region is conserved among many species including mouse, rat, chicken, nematode, fruit fly, and in JNK3.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is reactive to human and rat JNK1&2. Other species of JNK1&2 have not been tested, and JNK3 (found primarily in neuronal cell lines) has not been detected. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated JNK1&2. The final product is generated by affinity chromatography using a JNK1&2-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. Positive controls used: HEK 293 +/- UV irradiation treatment; PC12 cells +/- sorbitol.
Target Information
C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-jun kinase; c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; JNK 55; JNK-46; JNK-55; JNK/SAPK alpha; JNK1 beta1 protein kinase; Jun kinase; JUN N-terminal kinase; MAP kinase 8; MAP kinase 9; MAPK; MAPK 8; MAPK 9; MAPK8; MAPK9; mitogen activated protein kinase 8; mitogen activated protein kinase 9; Mitogen-activated protein kinase 8; Mitogen-activated protein kinase 9; OTTHUMP00000019552; p54 gamma; p54-alpha; Phospho-JNK; PRKM; protein kinase JNK1; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK 1; SAPK gamma; SAPK-alpha; SAPK1a; SAPK1c; stress activated protein kinase alpha II; stress-activated protein kinase 1; Stress-activated protein kinase 1a; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2; unnamed protein product
Gene Aliases: AI849689; AI851083; JNK; JNK-46; JNK-55; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAPK8; MAPK9; p54a; p54aSAPK; PRKM8; PRKM9; SAPK; SAPK1; SAPK1A; SAPK1C
UniProt ID: (Human) P45983, (Human) P45984, (Rat) P49185, (Mouse) Q91Y86, (Rat) P49186, (Mouse) Q9WTU6
Entrez Gene ID: (Human) 5599, (Human) 5601, (Rat) 116554, (Mouse) 26419, (Rat) 50658, (Mouse) 26420
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
JUN kinase activity
MAP kinase activity
protein binding
ATP binding
kinase activity
transferase activity
enzyme binding
protein phosphatase binding
histone deacetylase regulator activity
histone deacetylase binding
protein serine kinase activity
protein serine/threonine kinase binding
protein serine/threonine/tyrosine kinase activity
transferase activity, transferring phosphorus-containing groups
transcription factor binding
cysteine-type endopeptidase activator activity involved in apoptotic process
mitogen-activated protein kinase kinase kinase binding
non-receptor serine/threonine protein kinase
MAPK cascade
double-strand break repair
protein phosphorylation
response to oxidative stress
JNK cascade
JUN phosphorylation
response to UV
response to mechanical stimulus
positive regulation of gene expression
regulation of macroautophagy
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
positive regulation of cyclase activity
positive regulation of cell killing
negative regulation of protein binding
regulation of protein localization
cellular response to amino acid starvation
cellular response to oxidative stress
cellular response to reactive oxygen species
Fc-epsilon receptor signaling pathway
regulation of circadian rhythm
positive regulation of apoptotic process
negative regulation of apoptotic process
regulation of mRNA stability
NLRP3 inflammasome complex assembly
rhythmic process
positive regulation of protein metabolic process
stress-activated MAPK cascade
mRNA destabilization
protein poly-ADP-ribosylation
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
cellular senescence
energy homeostasis
positive regulation of NLRP3 inflammasome complex assembly
positive regulation of establishment of protein localization to mitochondrion
protein localization to site of double-strand break
positive regulation of macrophage derived foam cell differentiation
positive regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
response to cadmium ion
modulation of synaptic transmission
protein localization to tricellular tight junction
positive regulation of podosome assembly
inflammatory response to wounding
apoptotic signaling pathway
positive regulation of cytokine production involved in inflammatory response
positive regulation of apoptotic signaling pathway
ossification
regulation of transcription, DNA-templated
inflammatory response
response to osmotic stress
signal transduction
response to heat
regulation of gene expression
phosphorylation
positive regulation of cell migration
regulation of histone deacetylation
positive regulation of microtubule polymerization
neuron projection development
response to hydrogen peroxide
positive regulation of DNA replication
determination of dorsal identity
cellular response to hydrogen peroxide
cellular response to nitric oxide
positive regulation of deacetylase activity
type B pancreatic cell apoptotic process
neuronal stem cell population maintenance
programmed necrotic cell death
positive regulation of determination of dorsal identity
release of cytochrome c from mitochondria
positive regulation of protein phosphorylation
protein targeting to mitochondrion
activation of cysteine-type endopeptidase activity involved in apoptotic process
central nervous system development
response to toxic substance
response to organic substance
positive regulation of cell morphogenesis involved in differentiation
response to amine
positive regulation of prostaglandin biosynthetic process
regulation of protein ubiquitination
positive regulation of prostaglandin secretion
positive regulation of chemokine production
cellular response to UV
response to drug
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription, DNA-templated
regulation of JNK cascade
positive regulation of nitric-oxide synthase biosynthetic process
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to growth factor stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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