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Invitrogen
Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody
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FIGURE: 1 / 20
Phospho-p38 MAPK (Thr180, Tyr182) Antibody (44-684G) in ICC/IF
Immunofluorescent analysis of Phospho-P38 pThr180/pTyr182 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-P38 pThr180/pTyr182 Antibody (Product # 44-684G) at 1µg/mL in 1% BSA and incubated f... View More
Product Details
44-684G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human p38 that contains threonine 180 and tyrosine 182. This region is conserved among many species including mouse, rat, dog, monkey and carp.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with human and rat (100% homologous) p38 alpha, beta, and gamma. HEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol were used as positive controls.
Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182.
Target Information
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; EC 2.7.11.24; encoded by transcript variant 4; p38 MAP kinase; encoded by transcript variant 5; p38 MAP kinase; ERK1; ERT2; MAP 14; MAP kinase 1; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38alpha; MAPK 14; MAPK1 antibody; MAPK14; MAX-interacting protein 2; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38 alpha; MK14; OTTHUMP00000216563; OTTHUMP00000216566; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; reactive kinase; RP1-179N16.5; SAPK2a; SAPK2a; CSAIDS binding protein 1; Stress-activated protein kinase 2a; unnamed protein product
Gene Aliases: CRK1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; MAPK14; MXI2; p38; p38ALPHA; p38Hog; PRKM14; PRKM15; RK; SAPK2A
UniProt ID: (Human) Q16539
Entrez Gene ID: (Human) 1432, (Rat) 81649
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
MAP kinase kinase activity
protein binding
ATP binding
kinase activity
transferase activity
enzyme binding
protein phosphatase binding
mitogen-activated protein kinase p38 binding
NFAT protein binding
protein serine kinase activity
protein C-terminus binding
non-receptor serine/threonine protein kinase
DNA damage checkpoint
MAPK cascade
cell morphogenesis
cartilage condensation
angiogenesis
osteoblast differentiation
placenta development
response to dietary excess
chondrocyte differentiation
negative regulation of inflammatory response to antigenic stimulus
glucose metabolic process
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
apoptotic process
chemotaxis
cellular response to DNA damage stimulus
signal transduction
cell surface receptor signaling pathway
transmembrane receptor protein serine/threonine kinase signaling pathway
skeletal muscle tissue development
positive regulation of gene expression
positive regulation of myotube differentiation
fatty acid oxidation
platelet activation
regulation of ossification
osteoclast differentiation
stress-activated protein kinase signaling cascade
positive regulation of cyclase activity
lipopolysaccharide-mediated signaling pathway
response to muramyl dipeptide
response to lipopolysaccharide
positive regulation of interleukin-12 production
response to insulin
tumor necrosis factor-mediated signaling pathway
negative regulation of hippo signaling
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
response to muscle stretch
p38MAPK cascade
positive regulation of protein import into nucleus
signal transduction in response to DNA damage
positive regulation of blood vessel endothelial cell migration
positive regulation of erythrocyte differentiation
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
glucose import
positive regulation of glucose import
vascular endothelial growth factor receptor signaling pathway
stem cell differentiation
striated muscle cell differentiation
positive regulation of muscle cell differentiation
stress-activated MAPK cascade
protein maturation
positive regulation of cardiac muscle cell proliferation
bone development
pyroptosis
3'-UTR-mediated mRNA stabilization
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to tumor necrosis factor
cellular response to ionizing radiation
cellular response to UV-B
negative regulation of canonical Wnt signaling pathway
positive regulation of brown fat cell differentiation
cellular senescence
stress-induced premature senescence
cellular response to virus
regulation of synaptic membrane adhesion
regulation of cytokine production involved in inflammatory response
positive regulation of myoblast fusion
NLRP1 inflammasome complex assembly
positive regulation of reactive oxygen species metabolic process
transcription, DNA-templated
protein phosphorylation
response to glucose
myoblast differentiation involved in skeletal muscle regeneration
peptidyl-serine phosphorylation
protein autophosphorylation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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