Customer Notice: Please be sure to follow the primer concentration guidelines as specified in the User Guide. For example, if you are using SYBR® Select with a StepOne™ or StepOnePlus™ Real-Time PCR Instrument under Standard Mode, please use a primer concentration less than 200 nM. Also, for these two instruments, please note that new StepOne™ Software v2.3 is also available for download.

SYBR® Select Master Mix

Catalog number: 4472920

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.

Features of the SYBR® Select Master Mix include:

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility
SYBR® Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.

View performance data.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): 7500 System, 7500 Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode, StepOne™, Standard Mode, QuantStudio™ 12K Flex, ViiA™ 7 System, StepOnePlus™, Standard Mode
Reaction Speed: Standard, Fast
PCR Method: qPCR
Sample Type (General): DNA
Form: Liquid
Format: Vial(s)
Hot Start: Built-In Hot Start
Polymerase: AmpliTaq® DNA Polymerase
Label or Dye: SYBR® GreenER™
Product Size: 10 x 5 mL
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (Specific): cDNA, DNA (Genomic)
Purity or Quality Grade: UP (Ultra Pure)
Primer-Probe Compatibility: Unlabeled Oligos

Contents & storage

The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 10 X 5 mL tube, sufficient for 5000, 20 µL reactions (10 µL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

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