SYBR® Select Master Mix for CFX

Catalog number: 4472937

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

SYBR® Select Master Mix for CFX is a superior and cost-effective solution for your real-time PCR applications.

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
• Standard and fast cycling parameters

Formulated for Maximum Specificity
SYBR® Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix for CFX is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye, resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

View performance data.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): Bio-Rad CFX96 Touch™, 7500 Fast System, StepOnePlus™, Fast Mode, StepOne™, Standard Mode, ViiA™ 7 System, QuantStudio™ 12K Flex, StepOnePlus™, Standard Mode, StepOne™, Fast Mode, 7900HT Fast System, 7500 System, Bio-Rad CFX Connect™, 7900HT System, Bio-Rad CFX384 Touch™
Reaction Speed: Standard, Fast
PCR Method: qPCR
Sample Type (General): DNA
Form: Liquid
Format: Vial(s)
Hot Start: Built-In Hot Start
Polymerase: AmpliTaq® DNA Polymerase
Label or Dye: SYBR® GreenER™
Product Size: 1 x 1 mL
Shipping Condition: Wet Ice
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (Specific): cDNA, DNA (Genomic)
Purity or Quality Grade: UP (Ultra Pure)
Primer-Probe Compatibility: Unlabeled Oligos

Contents & storage

The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100 reactions at 20 µL. Store at 2-8°C.

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