SYBR™ Select Master Mix for CFX, 1 x 5 mL - FAQs

How does the SYBR GreenER Dye Chemistry work?

The SYBR GreenER dye binds to double stranded DNA formed during PCR. During PCR, the polymerase amplifies the target sequence which creates the amplicon. As PCR continues, the dye binds to each new copy of double-stranded DNA, leading to more amplicons being created. The result is an increase in fluorescence intensity proportional to the amount of double-stranded PCR product produced.