Applied Biosystems™

SYBR™ Select Master Mix for CFX

Catalog number:  4472952

 Related applications:

Real Time PCR (qPCR)

| Real Time PCR-Based Gene Expression Profiling

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix for CFX and added additional capabilities for your gene expression analysis.

SYBR® Select Master Mix for CFX is a superior and cost-effective solution for your real-time PCR applications.

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
• Standard and fast cycling parameters

Formulated for Maximum Specificity
SYBR® Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix for CFX is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye, resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control*

View performance data.

*Master mixes containing UDG should not be used with bisulfite-treated DNA samples.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: SYBR
For Use With (Equipment): Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™
Reaction Speed: Fast, Standard
PCR Method: qPCR
Sample Type (General): DNA
Form: Liquid
Format: Vial(s)
Hot Start: Built-In Hot Start
Label or Dye: SYBR® GreenER™
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq® DNA Polymerase
Primer-Probe Compatibility: Unlabeled Oligos
Product Line: SYBR®
Product Size: 2 x 5 mL
Purity or Quality Grade: UP (Ultra Pure)
Sample Type (Specific): DNA (Genomic), cDNA

Contents & storage

The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components. Contains 2 X 5 mL bottles, sufficient for 1000 reactions at 20 µL. Store at 2-8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols