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Invitrogen
Phospho-Tau (Ser262) Polyclonal Antibody
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FIGURE: 1 / 43
Phospho-Tau (Ser262) Antibody (44-750G) in IHC (P)
Immunohistochemistry analysis of Phospho-Tau (pS262) showing staining in the cytoplasm of paraffin-embedded human astroglioma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at r... View More
Product Details
44-750G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
Dog,
Fruit fly,
Human,
Mouse,
Rat,
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 262.
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Tau. The final product is generated by affinity chromatography using a Tau-derived peptide that is phosphorylated at serine 262. Positive control used: Recombinant human Tau +/- PKA, or African green monkey kidney (CV-1) cells, stably expressing human four repeat Tau and SV40 small T antigen to induce specific inhibition of PP2A.
Target Information
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; Microtubule-associated protein tau; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau microtubule-associated protein; Tau-4; Tau-derived paired helical filament hexapeptide; Tau5; unnamed protein product
Gene Aliases: AI413597; AW045860; DDPAC; FTD1; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; pTau; RNPTAU; TAU; tau-40; Tau-PHF6
UniProt ID: (Human) P10636, (Mouse) P10637
Entrez Gene ID: (Human) 4137, (Rat) 29477, (Mouse) 17762
DNA binding
AT DNA binding
double-stranded DNA binding
single-stranded DNA binding
RNA binding
actin binding
protein binding
microtubule binding
tubulin binding
SH3 domain binding
enzyme binding
protein kinase binding
protein binding, bridging
apolipoprotein binding
dynactin binding
phosphatidylinositol binding
identical protein binding
sequence-specific DNA binding
chaperone binding
protein phosphatase 2A binding
Hsp90 protein binding
lipoprotein particle binding
histone-dependent DNA binding
microtubule lateral binding
phosphatidylinositol bisphosphate binding
protein domain specific binding
protein complex binding
microtubule cytoskeleton organization
microglial cell activation
cellular response to DNA damage stimulus
cell-cell signaling
synapse assembly
learning or memory
memory
response to lead ion
regulation of autophagy
negative regulation of gene expression
negative regulation of mitochondrial membrane potential
rRNA metabolic process
axonal transport of mitochondrion
central nervous system neuron development
regulation of microtubule polymerization or depolymerization
regulation of microtubule polymerization
positive regulation of microtubule polymerization
cytoplasmic microtubule organization
neuron projection development
positive regulation of superoxide anion generation
regulation of chromosome organization
stress granule assembly
cellular response to heat
cellular response to reactive oxygen species
positive regulation of axon extension
microtubule polymerization
astrocyte activation
regulation of synaptic plasticity
intracellular distribution of mitochondria
generation of neurons
synapse organization
regulation of calcium-mediated signaling
protein polymerization
protein homooligomerization
axon development
regulation of microtubule cytoskeleton organization
plus-end-directed organelle transport along microtubule
regulation of mitochondrial fission
negative regulation of mitochondrial fission
fibril organization
axonal transport
regulation of cellular response to heat
regulation of long term synaptic depression
positive regulation of protein localization to synapse
neurofibrillary tangle assembly
negative regulation of establishment of protein localization to mitochondrion
positive regulation of cellular protein localization
negative regulation of tubulin deacetylation
amyloid fibril formation
cellular response to nerve growth factor stimulus
cellular response to brain-derived neurotrophic factor stimulus
neuron migration
apoptotic process
microtubule-based process
axonogenesis
brain development
female pregnancy
response to nutrient
adult walking behavior
axo-dendritic transport
intrinsic apoptotic signaling pathway in response to oxidative stress
response to organic substance
neuron differentiation
negative regulation of intracellular transport
mitochondrion transport along microtubule
axon extension
regulation of microtubule-based movement
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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