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Invitrogen
Phospho-SHC (Tyr239, Tyr240) Polyclonal Antibody
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FIGURE: 1 / 10
Phospho-SHC (Tyr239, Tyr240) Antibody (44-830) in IHC (P)
Immunohistochemistry analysis of SHC (PYPY239/240) showing staining in the cytoplasm and nucleus of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 m... View More
Product Details
44-830
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Shc that contains tyrosines 239 and 240.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ26504; OTTHUMP00000035472; RP11-307C12.1; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; unnamed protein product
Gene Aliases: p66; p66shc; SHC; SHC1; SHCA
UniProt ID: (Human) P29353, (Mouse) P98083
Entrez Gene ID: (Human) 6464, (Mouse) 20416
phosphotyrosine binding
transmembrane receptor protein tyrosine kinase adaptor activity
epidermal growth factor receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
phospholipid binding
receptor tyrosine kinase binding
ephrin receptor binding
epidermal growth factor binding
protein kinase binding
protein complex binding
phosphoprotein binding
protein phosphatase 2A binding
scaffold/adaptor protein
angiogenesis
epidermal growth factor receptor signaling pathway
heart development
positive regulation of cell proliferation
insulin receptor signaling pathway
negative regulation of angiogenesis
actin cytoskeleton organization
intracellular signal transduction
regulation of cell proliferation
defense response to bacterium
negative regulation of apoptotic process
positive regulation of MAPK cascade
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
insulin-like growth factor receptor signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to growth factor stimulus
positive regulation of cell proliferation in bone marrow
regulation of superoxide metabolic process
cell-cell adhesion
MAPK cascade
activation of MAPK activity
signal transduction
aging
single organismal cell-cell adhesion
neuron differentiation
neuron projection development
actin cytoskeleton reorganization
regulation of growth
positive regulation of DNA replication
positive regulation of vasoconstriction
positive regulation of smooth muscle cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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