Beginning August 15, 2014, this product will ship under ambient conditions. Full details can be found here.
Applied Biosystems™
TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe, primer-limited
Error loading your content!
| Catalog number |
Select a plan
Unit size
|
Price ({{currency}}) | Availability | Qty | |
|---|---|---|---|---|---|
| {{product.sku}} {{product.sku}} also known as {{product.formattedSku}} |
|
— |
Pro add-ons
Your on-site stock
››
{{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
››
{{supplyCenter.scName}}(Out of stock)
››
{{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Description
The Applied Biosystems® TaqMan® GAPDH Assay with JUN® dye-labeled QSY® probe (primer limited) provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of a JUN® dye-labeled QSY® probe plus sequence-specific forward and reverse primers. The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress GAPDH.Benefits of this assay include:
• Eliminate assay design time by using pre-designed primers and pre-designed JUN® dye-labeled TaqMan® probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC® dye-labeled predesigned gene expression assays
• Formulated at a primer-limited concentration for use in multiplex reactions
Save time in assay design, formulation, and testing by using the TaqMan® GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.
In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high GAPDH expressors.
The JUN® dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN® dye) due to an overlap of spectra. TaqMan® Multiplex Master Mix with MUSTANG PURPLE® dye is recommended.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Accession Number: | NM_002046.3 |
|---|---|
| Amplicon Size: | 118 |
| Format: | Tube(s) |
| Gene Symbol: | GAPDH |
| Includes: | Primer-Probe Set(s) |
| Internal Probe Modification: | QSY® Non-Fluorescent Quencher (3') |
| Label or Dye: | JUN™ |
| Product Line: | TaqMan™ |
| Product Size: | 250 reactions |
| Species: | Human |
| Target Gene: | Glyceraldehyde-3-phosphate dehydrogenase |
| Green Features: | Sustainable packaging |
Contents & storage
Store in freezer (-5 to -30°C).
