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Invitrogen
MTCO1 Monoclonal Antibody (1D6E1A8)
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FIGURE: 1 / 57
MTCO1 Antibody (459600) in ICC/IF
Immunofluorescence analysis of MTCO1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MTCO1 Monoclonal Antibody (1D6E1A8) (Product # 459600) at 5 µg/mL dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produc... View More
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.jpg?time=20220826?time=20220826 "MTCO1 Antibody (459600) in WB")
Product Details
459600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Arthropod,
Guinea pig,
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
1D6E1A8
Immunogen
Human Mitochondrial Complex IV subunit I
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.99 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 0.05% BSA, 40% glycerol
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal. Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio. In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa). In mouse liver lysate a specific band below 37 kDa was detected.
Recommended positive controls:
ICC/IF - Pig retinal pigment epithelial cells, rat cerebellum primary cells
IHC (P) - Human skeletal muscle and colon tissue, rat pancreas tissue, pig smooth muscle tissue
WB - Mouse, human, bovine and rat heart mitochondria lysate
Flow - HEK-293 cells
Recommended long term storage at -20°C.
Target Information
Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Co1; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1
Gene Aliases: Coi; CoxI; Mt-co1; Mtco1
Entrez Gene ID: (Mouse) 17708
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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