DyLight™ 488 Maleimide

Catalog number: 46602

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

Thermo Scientific DyLight 488 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 488 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes.

DyLight 488 has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than Cy2™ dye, Alexa Fluor™ 488, FITC, and LI-COR™ dyes in many applications. The high water solubility of DyLight Fluors allows a high dye-to-protein ratio to be achieved without causing precipitation of the conjugates.

Features of DyLight 488 Maleimide:

High performance—DyLight 488 shows brighter fluorescence than Alexa Fluor 488, FITC and Cy2 dye
Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies

DyLight 488 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5–7.5. Learn more about maleimide chemistry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity: Thiol
Excitation⁄Emission (nm): 493⁄518
Label or Dye: DyLight™ 488
Product Line: DyLight™
Product Size: 1 mg
Solubility: DMF (Dimethylformamide)

Contents & storage

Store desiccated at -20°C. Shipped at ambient temperature.

Documents

Manuals & protocols